Developmental biology protocols. / Volume II

Drosophila as a genetic tool to define vertebrate pathway players / N.M. Bonini -- Bioassays for studying the role of the peptide growth factor activin in early amphibian embryogenesis / M. Asashima ... [et al.] -- Analysis of mammary gland morphogenesis / C.D. Roskelley, C. Wu and A.M. Somasiri -- Specification of cardiac mesenchyme and heart morphogenesis in vitro / H.J. Yost -- Craniofacial development and patterning / H. Slavkin, G. Nuckolls and L. Shum -- Craniofacial skeletal morphogenesis in vitro / R.C. Ogle -- Skeletal morphogenesis / S. Mundlos -- Transplantation and culture techniques for the analysis of urodele limb regeneration / D.L. Stocum -- Retroviral infection of t-cell precursors in thymic organ culture / L.M. Spain, L.L. Lau and Y. Takahama -- Assay for the isolation of hepatogenic factors. Key molecules in hepatocyte formation and liver morphogenesis / J.A. Spijkers, T.B. Hakvoort and W.H. Lamers -- Skin morphogenesis. Embryonic chicken skin explant cultures / C.M. Chuong -- Apoptosis in development / L. Lin and Z.F. Zakeri -- Methods to detect patterns of cell death in drosophila / N.M. Bonini -- Mechanisms of neurulation / G.C. Schoenwolf and J.L. Smith -- Neurulation and neural tube closure defects / A. Copp ... [et al.] -- Neural tube defects / T. Inagaki ... [et al.] -- Experimental manipulation and morphometric analysis of neural tube development / M.E. Desmond and P.A. Haas -- Isolation of neuroepithelium and formation of minispheres / M.E. Desmond and M.C. Field -- Examination of normal and abnormal placentation in the mouse / M.R. Blackburn -- Palatal dysmorphogenesis. Palate organ culture / B.D. Abbott -- Palatal dysmorphogenesis. Quantitative rt-pcr / G.A. Held and B.D. Abbott -- Transspecies grafting as a tool to understand the basis of murine developmental limb abnormalities / S.M. Bell, C.M. Schreiner and W.J. Scott -- Assessment of laterality defects in rodent embryos / M. Fujinaga -- Cardiac morphogenesis and dysmorphogenesis. I. Normal development / A. Wessels and R. Markwald -- Analysis of two aspects of left-right patterning of the vertebrate heart. Heart tube position and heart tube chirality / A.J. Chin -- Biologically based risk assessment models for developmental toxicity / C. Lau and R.W. Setzer -- Positional cloning / L. Xu and Y. Li -- Gene trapping in embryonic stem cells in vitro to identify novel developmentally regulated genes in the mouse / G.E. Lyons ... [et al.] -- PCR-based cloning of cortically localized rnas from xenopus oocytes / J. Zhang and M.L. King -- Analysis of mRNA expression during preimplantation development / K.E. Latham, E. De la Casa and R.M. Schultz -- Differential screens with subtracted pcr-generated cDNA libraries from subregions of single mouse embryos / A. Shimono and R.R. Behringer -- HPLC-based mRNA differential display / T.B. Knudsen -- Production of transgenic drosophila / M. Fujioka ... [et al.] -- Transgenic manipulation of the sea urchin embryo / J.P. Rast -- Transgenic zebrafish / S. Lin -- Production of avian chimeras and germline transmission / K.L. Woods ... [et al.] -- Incorporation of genetically modified cells in chicken chimeras / L.D. Zajchowski ... [et al.] -- Long-term culture of chicken blastodermal cells (cbcs) and selection of transfected cbcs using antibiotic resistance / Q. Wei, K.L. Woods and R.J. Etches -- Nuclear transplantation and cloning in mammals / K.E. Latham and M.E. Westhusin -- Production of transgenic mice / J. Richa -- Production of transgenic mice with yeast artificial chromosomes / A. Jakobovits, B.T. Lamb and K.R. Peterson -- Gene-targeting strategies / S.S. Cheah and R.R. Behringer -- Chimeric animals and germline transmission / J.S. Khillan -- Conditional gene knockout using cre recombinase / Y. Le and B. Sauer -- Application of cre/loxp in drosophila. Site-specific recombination and transgene coplacement / M.L. Siegal and D.L. Hartl