Developmental biology protocols. / Volume III

Ectopic expression in drosophila / E.L. Wilder -- Clonal analysis in the examination of gene function in drosophila / J.E. Rooke, N.A. Theodosiou and T. Xu -- Application of antisense oligodeoxynucleotides in developing chick embryos / P.G. Alexander, G.L. Barnes and R.S. Tuan -- Application of functional blocking antibodies. N-cadherin and chick embryonic limb development / S.A. Oberlender and R.S. Tuan -- Gene expression analyzed by ribonuclease protection assay / V.D. Bennett -- Relative reverse transcription-polymerase chain reaction / T.A. Giambernardi and R.J. Klebe -- Gene expression analysis using quantitative reverse transcription-polymerase chain reaction and a multispecific internal control / D. Shire and P. Legoux -- In situ pcr detection of hiv expression in the human placenta / A.U. Sheikh, B.M. Polliotti and R.K. Miller -- Gene expression analysis by in situ hybridization. Radioactive probes / S. Wawersik and J.A. Epstein -- Radio-isotopic in situ hybridization on tissue sections. Practical aspects and quantification / A.F. Moorman ... [et al.] -- mRNA and protein co-localization on tissue sections by sequential, colorimetric in situ hybridization and immunohistochemistry / R.S. Tuan -- Whole mount in situ hybridization to study gene expression during mouse development / L.A. Lowe and M.R. Kuehn -- Multicolor whole-mount in situ hybridization / G. Hauptmann and T. Gerster -- Methods for double detection of gene expression. Combined in situ hybridization and immunocytochemistry of histochemistry / R.A. Conlon -- Visualization of the expression of green fluorescent protein (GFP)-linked proteins / J.C. Ayoob, J.M. Sanger and J.W. Sanger -- Monoclonal antibodies in the analysis of embryonic development / V. Bedian -- Mesoderm induction in Xenopus oocyte expression system and animal cap assay / J. Yao and D.S. Kessler -- Amphibian organizer activity / C. Niehrs -- Improved techniques for avian embryo culture, somite cell culture, and microsurgery / D.S. Packard, Jr., C. Cox and T.J. Poole -- Neural crest cell outgrowth cultures and the analysis of cell migration / D.F. Newgreen and M. Murphy -- The chimeric human/mouse model of angiogenesis / E. Petitclerc, T. von Schalscha and P.C. Brooks -- Analysis of embryonic vascular morphogenesis / T.N. Sato and S. Bartunkova -- Epithelial-mesenchymal interactions / B.K. Hall -- Methods for manipulating the chick limb bud to study gene expression, tissue interactions, and patterning / M.A. Ros ... [et al.] -- Palate development. In vitro procedures / M.M. Pisano and R.M. Greene -- In vitro fertilization / S. Heyner and M.J. Tucker -- Trophoblast differentiation. An in vitro model for trophoblast giant cell development / T.J. Peters, B.M. Chapman and M.J. Soares -- Bone marrow-derived mesenchymal progenitor cells / B. Johnstone and J. Yoo -- Identification, characterization, and differentiation of human prostate cells / P.P. Mehta ... [et al.] -- Preparation of chick striated muscle cultures / C. DiLullo, J. Gerhart and M. George-Weinstein -- Study of skeletal myogenesis in cultures of unsegmented paraxial mesoderm / A.G. Borycki and C.P. Emerson, Jr. -- Embryonic limb mesenchyme micromass culture as an in vitro model for chondrogenesis and cartilage maturation / A.M. DeLise ... [et al.] -- Electroporation-mediated DNA transfection of embryonic chick limb mesenchymal cells / A.M. DeLise and R.S. Tuan -- Murine C3H10T1/2 multipotential cells as an in vitro model of mesenchymal chondrogenesis / A.R. Haas and R.S. Tuan -- Skeletogenesis. In vitro analysis of bone cell differentiation / A. Majolagbe and P.G. Robey -- Studying early hematopoiesis using avian blastoderm cultures / C.A. Eisenberg -- Isolation and culture of mouse germ cells / M.P. De Miguel and P.J. Donovan -- Cadherin-mediated cell-cell interactions / K.A. Knudsen and A.P. Soler -- Analysis of hyaluronan using biotinylated hyaluronan-binding proteins / C.B. Underhill and L. Zhang -- Microinjection of fluorescently labeled alpha-actinin into living cells / J.M. Sanger, B.A. Danowski and J.W. Sanger -- Pax3 and vertebrate development / J.A. Epstein -- Genetic-engineered models of skeletal diseases. I. Collagen type X / O. Jacenko -- Genetic-engineered models of skeletal diseases. II. Targeting mutations into transgenic mice chondrocytes / S. Garofalo and W.A. Horton -- Transgenic mouse models of craniofacial disorders / Y.H. Liu, M.L. Snead and R.E. Maxson, Jr.