Adhesion protein protocols

Monoclonal antibodies specific for leukocyte adhesion molecules : selective protocols of immunization and screening assays for generation of blocking, activating and activation reporter antibodies / Carlos Cabañas, Francisco Sánchez-Madrid -- Epitope mapping / Horace M. DeLisser -- Sequencing of antibodies / Annie Jarrin, Annie Andrieux -- Purification through affinity chromatography and microsequencing / Cecilia Garlanda -- Functional analysis of cell adhesion molecules / David L. Simmons -- Application of polymerase chain reaction for the discovery of new adhesion molecule family members / Amy S. Woodard, Lucia R. Languino -- Construction and purification of adhesion molecule immunoglobulin chimeric proteins / Thomas Moll, Dietmar Vestweber -- Immunofluorescence of cultured cells / Pier Carlo Marchisio, Livio Trusolino -- Detection of adhesion molecules by immunohistochemistry on human and murine tissue sections / Antonella Stoppacciaro, Luigi P. Ruco -- In situ hybridization with RNA probes / Georg Breier -- Purification of fibronectin from human plasma / Saverio Francesco Retta, Piercarlo Ferraris, Guido Tarone -- Adhesion to matrix proteins / Saverio Francesco Retta, Massimo Ternullo, Guido Tarone -- Leukocyte-endothelial monolayer adhesion assay (static conditions) / Jeanne-Marie Kiely, Francis W. Luscinskas, Michael A. Gimbrone -- Cell-cell adhesive interactions in an in vitro flow chamber / Douglas J. Goetz, Daniel M. Greif, Jian Shen, Francis W. Luscinskas -- Adhesion of tumor cells to endothelium under static conditions / Renato G. Chiarivi, Raffaella Giavazzi -- Adhesion of tumor cells under flow / Andrea Remuzzi, Raffaella Giavazzi -- Platelet adhesion to the subendothelium under flow / Philip G. Groot, Martin J. IJsseldijk, Jan J. Sixma -- Leukocyte transmigration through vascular endothelium : an in vitro method / Paola Allavena, Aldo Maschio -- Cell migration into a wounded area in vitro / Maria Grazia Lampugnani -- Determination of the endothelial barrier function in vitro / Geerten P. Nieuw Amerongen, Victor W. Hinsbergh -- Induction of intracellular signals through binding of adhesive molecules : activation of p125FAK tyrosine phosphorylation / Mascia Venturino, Cristina Olivo, Guido Tarone, Paola Defilippi -- Induction of Ca2+ flux by adhesion molecules in lymphocytes / Ruggero Maria, Gabriella Palmieri, Angela Santoni -- Integrin-mediated stimulation of tyrosine phosphorylation in lymphoid cells / Angela Gismondi, Angela Santoni -- The identification of signaling molecules by the yeast two-hybrid system / Stefano Biffo, Pier Carlo Marchisio