Germ cell protocols. / Volume 1, Sperm and oocyte analysis

Cryopreservation of semen of the salmonidae with special reference to large-scale fertilization / F. Lahnsteiner -- Fertilization and sperm chemotaxis in ascidians / M. Yoshida -- Assays for vertebrate sperm chemotaxis / H. Sugiyama ... [et al.] -- Stallion spermatozoa viability: Comparison of flow cytometry with other methods / K. Merkies and M.M. Buhr -- Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy / P. Sutovsky -- Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins / H.R. Burkin, A. Paula Alves-Vieira and D.J. Miller -- Detection of human papillomavirus DNA in sperm using polymerase chain reaction / O.A. Olatunbosun, A.M. Case and H.G. Deneer -- Spermatogonial stem cells / K. Nayernia, M. Li and W. Engel -- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system / K. Nayernia and W. Engel -- Acrosin activity assay for the evaluation of mammalian sperm acrosome reaction / Y. Lax, S. Rubinstein and H. Breitbart -- Isolation of sea urchin sperm plasma membranes / K.J. Mengerink and V.D. Vacquier -- Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy / H. Schatten and A. Chakrabarti -- Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development / M.W. Tengowski -- Immunocytochemical staining of the metaphase ii spindle in mammalian oocytes / S.F. Mullen and J.K. Critser -- Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use / W.H. Wang, J. Gill and C. Boutin -- In vitro maturation and fertilization of pig oocytes / H.Y. Fan and Q.Y. Sun -- In vitro maturation and in vitro fertilization of human oocytes / W.H. Wang and J. Gill -- In vitro maturation and fertilization of canine oocytes / K. Reynaud, M. Saint-Dizier and S. Chastant-Maillard -- Detection and measurement of membrane-bound protein tyrosine kinases in the zebrafish egg / W. Wu and W.H. Kinsey -- Single-cell method for estimation of protein tyrosine kinases in the zebrafish egg / E. MacGregor and W.H. Kinsey -- Activity of MAPK/p90rsk during fertilization in mice, rats, and pigs / Q.Y. Sun and H.Y. Fan