Cyclooxygenases : methods and protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Samir S Ayoub; R J Flower; Michael P Seed
- ناشر : New York, NY : Humana Press : Springer Science+Business Media
- چاپ و سال / کشور: 2010
- شابک / ISBN : 9781597453646
Description
Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns -- 1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower -- 2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata -- 3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse -- 4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz -- 5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons -- 6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett -- 7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates -- 8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik -- 9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub -- 10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood -- 11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse -- -- Part II. Extraction and measurement of prostanoids and isoprostanes -- 12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani -- 13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub -- 14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani -- -- Part III. In vivo models to study involvement of cyclooxygenase products in health and disease -- 15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson -- 16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle -- 17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed -- 18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.
Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns --
1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower --
2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata --
3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse --
4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz --
5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons --
6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett --
7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates --
8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik --
9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub --
10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood --
11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse --
--
Part II. Extraction and measurement of prostanoids and isoprostanes --
12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani --
13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub --
14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani --
--
Part III. In vivo models to study involvement of cyclooxygenase products in health and disease --
15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson --
16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle --
17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed --
18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.
1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower --
2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata --
3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse --
4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz --
5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons --
6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett --
7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates --
8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik --
9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub --
10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood --
11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse --
--
Part II. Extraction and measurement of prostanoids and isoprostanes --
12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani --
13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub --
14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani --
--
Part III. In vivo models to study involvement of cyclooxygenase products in health and disease --
15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson --
16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle --
17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed --
18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.