Protein secretion : methods and protocols

The extraordinary diversity of bacterial protein secretion mechanisms / I.B. Holland -- In vitro and in vivo approaches to studying the bacterial signal peptide processing / P. Wang and R.E. Dalbey -- Membrane insertion of small proteins / A. Kuhn, N. Stiegler and A.K. Schubert -- Membrane protein insertion in E. coli / J. Yuan, R.E. Dalbey and A. Kuhn -- Study of polytopic membrane protein topological organization as a function of membrane lipid composition / M. Bogdanov, P.N. Heacock and W. Dowhan -- In vivo analysis of protein translocation to the Escherichia coli periplasm / D. Belin -- Sorting of bacterial lipoproteins to the outer membrane by the Lol system / S. Narita and H. Tokuda -- Purification and functional reconstitution of the bacterial protein translocation pore, the SecYEG complex / I. Kusters ... [et al.] -- Reconstitution of the SecY translocon in nanodiscs / K. Dalal and F. Duong -- In vitro assays to analyze translocation of the model secretory preprotein alkaline phosphatase / G. Gouridis ... [et al.] -- Characterization of interactions between proteins using site-directed spin labeling and electron paramagnetic resonance spectroscopy / J.M. Crane, A.A. Lilly and L.L. Randall -- Analysis of Tat targeting function and twin-arginine signal peptide activity in Escherichia coli / T. Palmer, B.C. Berks and F. Sargent -- Site-specific cross-linking of in vitro synthesized E. coli preproteins for investigating transmembrane translocation pathways / S. Panahandeh and M. Muller -- Tracking the secretion of fluorescently labeled type III effectors from single bacteria in real time / N. Simpson, L. Audry and J. Enninga -- Comparative analysis of cytoplasmic membrane proteomes of Escherichia coli using 2D blue native/SDS-PAGE / S. Schlegel ... [et al.] -- Using hidden markov models to discover new protein transport machines / V.A. Likic ... [et al.] -- Bioinformatics predictions of localization and targeting / S. Rastogi and B. Rost -- The chloroplast protein import machinery: a review / P. Strittmatter, J. Soll and B. Bolter -- Measurement of the energetics of protein transport across the chloroplast thylakoid membrane / S.M. Theg -- In vitro dissection of protein translocation into the mammalian endoplasmic reticulum / A. Sharma ... [et al.] -- in vitro reconstitution of the selection, ubiquitination, and membrane extraction of a polytopic ERAD substrate / K. Nakatsukasa and J.L. Brodsky -- Studying the ArfGAP-dependent conformational changes in SNAREs / F. Rodriguez and A. Spang -- Studying endoplasmic reticulum function in vitro using siRNA / C.M. Wilson and S. High -- High-quality immunofluorescence of cultured cells / D. Bhattacharyya, A.T. Hammond and B.S. Glick -- Trapping oxidative folding intermediates during translocation to the intermembrane space of mitochondria: in vivo and in vitro studies / D.P. Sideris and K. Tokatlidis -- Native techniques for analysis of mitochondrial protein import / F.N. Vogtle ... [et al.].