Plant secondary metabolism engineering : methods and applications

Plant secondary metabolism and challenges in modifying its operation : an overview / Naíla Cannes do Nascimento and Arthur Germano Fett-Neto -- Suppression subtractive hybridization as a tool to identify anthocyanin metabolism-related genes in apple skin / Yusuke Ban and Takaya Moriguchi -- Identification of regulatory protein genes involved in alkaloid biosynthesis using a transient RNAi system / Yasuyuki Yamada ... [et al.] -- Site-directed mutagenesis and saturation mutagenesis for the functional study of transcription factors involved in plant secondary metabolite biosynthesis / Sitakanta Pattanaik ... [et al.] -- Isolation of proteins binding to promoter elements of alkaloid metabolism-related genes using yeast one-hybrid / Débora Vom Endt and Giancarlo Pasquali -- Modulation of carotenoid accumulation in transgenic potato by inducing chromoplast formation with enhanced sink strength / Joyce Van Eck ... [et al.] -- Over-expression of rate-limiting enzymes to improve alkaloid productivity / Tomoya Takemura ... [et al.] -- Microbial expression of alkaloid biosynthetic enzymes for characterization of their properties / Hiromichi Minami, Nobuhiro Ikezawa, and Fumihiko Sato -- Producing a recombinant flavin-containing monooxygenase from Coffea arabica in Escherichia coli for screening of potential natural substrates / Igor Cesarino and Paulo Mazzafera -- Efficient production of active form recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture / Hisashi Semba ... [et al.] -- Introduction of the early pathway to taxol biosynthesis in yeast by means of biosynthetic gene cluster construction using SOE-PCR and homologous recombination / Pia Dahm and Stefan Jennewein -- Biocatalytic synthesis of tritium (³H)-labelled taxa-4(5), 11(12)-diene, the pathway committing precursor of the taxoid diterpenoids / Hans Schmeer and Stefan Jennewein -- USER cloning and USER fusion : the ideal cloning techniques for small and big laboratories / Hussam H. Nour-Eldin, Fernando Geu-Flores, and Barbara A. Halkier -- Enrichment of carotenoids in flaxseed by introducing a bacterial phytoene synthase gene / Masaki Fujisawa and Norihiko Misawa -- Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants / Tomohisa Hasunuma, Akihiko Kondo, and Chikahiro Miyake -- Engineering high yields of secondary metabolites in Rubia cell cultures through transformation with rol genes / Victor P. Bulgakov, yuri N. Shkryl, and Galina N. Veremeichik -- Flow cytometric methods to investigate culture heterogeneities for plant metabolic engineering / Vishal Gaurav, Martin E. Kolewe, and Susan C. Roberts -- Phenylpropanoid biosynthesis in leaves and glandular trichomes of basil (Ocimum basilicum L.) / Cícero Deschamps and James E. Simon -- Fusion with fluorescent proteins for subcellular localization of enzymes involved in plant alkaloid biosynthesis / Patrícia Duarte, Johan Memelink, and Mariana Sottomayor -- Immunohistochemical localisation of a putative flavonoid transporter in grape berries / Elisa Petrussa ... [et al.] -- Electrogenic bromosulfalein transport in isolated membrane vesicles : implementation of both animal and plant preparations for the study of flavonoid transporters / Sabina Passamonti ... [et al.]