Protein blotting and detection
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Biji T Kurien; R Hal Scofield
- ناشر : New York, NY : Humana Press
- چاپ و سال / کشور: 2009
- شابک / ISBN : 9781934115732
Description
Origins of protein blotting / H. Towbin -- Western blotting : remembrance of past things / W.N. Burnette -- Introduction to protein blotting / B.T. Kurien and R.H. Scofield -- Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis / D.Y. Lee and G.D. Chang -- Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support / Olsen and H.G. Wiker -- Tryptic peptide purification using polyvinylidene difluoride membrane for matrix-assisted laser desorption ionization time of flight mass spectrometry / B.T. Kurien and R.H. Scofield -- Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over time / B.T. Kurien and R.H. Scofield -- Nonelectrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens to obtain 12 immunoblots / B.T. Kurien and R.H. Scofield -- Slice blotting / G. Lowe -- Localizing proteins by tissue printing / R.F. Pont-Lezica -- Dot-immunobinding assay / S. Sumi, A. Mathai and V.V. Radhakrishnan -- Capillary blotting of glycosaminoglycans on nitrocellulose membranes after agarose-gel electrophoresis separation / N. Volpi and F. Maccari -- Quantitative computerized western blotting / D. Talmi-Frank, C.L. Jaffe and G. Baneth -- Cationic electrophoresis and eastern blotting / E. Buxbaum -- A miniaturized blotting system for simultaneous detecting of differentautoantibodies / U. Canzler ... [et al.] -- Transfer and multiplex immunoblotting of a paraffin embedded tissue / J.Y. Chung and S.M. Hewitt -- Multistrip Western blotting to increase quantitative data output / A. Kiyatkin and E. Aksamitiene -- Western blotting/Edman sequencing using PVDF membrane / S. Komatsu -- Blotting from PhastGel to membranes by ultrasound / J. Kost -- Ultrarapid electrophoretic transfer of high and low molecular weight proteins using heat / B.T. Kurien and R.H. Scofield -- Strip immunoblotting of multiple antigenic peptides to nitrocellulose membrane / B.T. Kurien -- Affinity purification of autoantibodies from an antigen strip excised from a nitrocellulose protein blot / B.T. Kurien -- Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures / F. Lasne -- Efficient electroblotting of very large proteins using a vertical agarose electrophoresis system / M.L. Greaser and C.M. Warren -- Semi-dry protein transfer and immunodetection of P-selectin using an antibody to its C-terminal tag / P. Mehta -- The multiple antigen blot assay: a simple, versatile and multipurpose immunoenzymatic technique / O. Noya ... [et al.] -- Blotting from immobilized pH gradient gels: Application to total cell lysates / H. Towbin -- Immunoprecipitation-Western blot for proteins of low abundance / E.P. Trieu, J.K. Gross and I.N. Targoff -- Native electrophoresis and Western blot analysis: method and applications / I.N. Manoussopoulos and M. Tsagris -- Grid-immunoblotting / O. Yeliosof and R.H. Scofield -- Sequential use of immunoblots for characterization of autoantibody specificities / H. Bartsch and M. Bachmann -- Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membrane / B.T. Kurien and M. Bachmann -- Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis / Y.T. Liu and S. Toyokuni -- Detection of protein-protein interactions by far-Western blotting / K. Machida and B.J. Mayer -- On-membrane tryptic digestion of proteins for mass spectrometry analysis / J.L. Luque-Garcia and T.A. Neubert -- Western blot analysis of adhesive interactions under fluid shear conditions: The blot rolling assay / R. Sackstein and R. Fuhlbrigge -- ELISPOT assay on membrane microplates / A.E. Kalyuzhny -- A brief review of other notable protein blotting methods / B.T. Kurien and R.H. Scofield -- Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore / L.B. Bockus and R.H. Scofield -- Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation / N.W. Charter ... [et al.] -- Fluorescent labeling of proteins and its application to SDS-PAGE and western blotting / F.J. Alba ... [et al.] -- Whole and strip nitrocellulose membrane as well as a new line immunoblotting of antigen using the chemiluminescence technique / Y. Dorri, A.W. Khalili and R.H. Scofield -- Protein stains to detect antigen on membranes / A. D'Souza and R.H. Scofield -- Use of non-radioactive detection method for North- and Southwestern blot / C. Franke ... [et al.] -- Immunoblotting using radiolabeled reagents for detection / H. Bartsch, C. Franke and M. Bachmann -- Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods / K. Hensley -- "Rainbow" Western blotting / S.S. Krajewski -- Multiple antigen detection (MAD) western blotting / S. Krajewski, X. Huang and M. Krajewska -- Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2 / B.T. Kurien and M. Bachmann -- Detection of La/SS-B by Western blot using nanogold-tagged antibodies and silver enhancement / S. Maier, S. Hubbell and R.H. Scofield -- Imaging systems for westerns: Chemiluminescence vs. infrared detection / S.T. Mathews, E.P. Plaisance and T. Kim -- A fluorescent codetection system for immunoblotting and proteomics through ECL-Plex and CyDye labeling / C.A. McManus, P.M. Donoghue and M.J. Dunn -- Visualization of unstained protein bands on PVDF / J. Park, M. Mabuchi and A. Sharma -- Improved protein detection using cold microwave technology / A.G. Smith ... [et al.] -- TLC blot (far-eastern blot) and its applications / T. Taki ... [et al.] -- A brief review of other notable protein detection methods on blots / B.T. Kurien and R.H. Scofield -- Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assays / B.T. Kurien.
The development of the Western blot has revolutionized biomedical research and medical diagnostics. This book offers numerous protein transfer and detection techniques based on the Western blot