Viral applications of green fluorescent protein : methods and protocols

1. Using fluorescent proteins to study poxvirus morphogenesis / Brian M. Ward -- 2. Application of reporter gene imaging for studying human embryonic stem cells in living subjects / Kazim H. Narsinh, Feng Cao, and Joseph C. Wu -- 3. Use of a GFP-PML-expressing cell line as a biosensor for human cytomegalovirus infection / Tomonori Ueno and Kiyoko Ogawa-Goto -- 4. Dual-color imaging of tumor angiogenesis / Robert M. Hoffman -- 5. Use of GFP to analyze morphology, connectivity, and function of cells in the central nervous system / Alan R. Harvey ... [et al.] -- 6. Expression of EGFP by adenovirus-mediated gene transfer in the central nervous system / Ryohei Tomioka -- 7. Adenovirus-mediated delivery of short hairpin RNA (shRNA) mediates efficient gene silencing in terminally differentiated cardiac myocytes / Andreas Rinne ... [et al.] -- 8. Microscopic analysis of adenoviral decontamination using GFP adenovirus with comparable sensitivity to flow cytometry / Michael Grusch ... [et al.] -- 9. Production of multicistronic HIV-1 based lentiviral vectors / Nachimuthu Chinnasamy, James Shaffer, and Dhanalakshmi Chinnasamy -- 10. Using an EGFPmeter to evaluate the lentiviral vector production: tricks and traps / Claude Bagnis, Pascal Bailly, and Sylvie Chapel-Fernandes -- 11. Use of a mcrophage-tropic GFP-tagged human immunodeficiency virus type 1 (HIV-1) to study viral reservoirs / Amanda M. Brown -- 12. GFP-lentiviral vectors targeting for neuroAIDS / Yuanan Lu -- 13. Construction of cell-type specific promoter lentiviruses for optically guiding electrophysiological recordings and for targeted gene delivery Aaron M. Jasnow ... [et al.] -- 14. Selective Protein Expression Within the CNS Using Hybrid Lentivirus / Fredrik Gussing ... [et al.] -- 15. Construction and analysis of alphaherpesviruses expressing green fluorescent protein / Bruce W. Banfield and Gregory A. Bird -- 16. The use of green fluorescent fusion proteins to monitor herpes simplex virus replication / Travis J. Taylor and David M. Knipe -- 17. Modified bovine herpesvirus 1 for protein secretion / Gunther M. Keil -- 18. Identification of viral peptide fragments for vaccine development / Zhanglin Lin, Shuang Li, and Yong Chen -- 19. Use of GFP to investigate expression of plant-derived vaccines / Hong-Ye Li and Mee-Len Chye -- 20. Studies of the role and function of barley stripe mosaic virus encoded proteins in replication and movement using GFP fusions / Sophie Haupt ... [et al.] -- 21. Sendai virus for cancer immunotherapy / Yasuji Ueda, Mamoru Hasegawa, and Yoshikazu Yonemitsu -- 22. Poxvirus tropism for primary human leukocytes and hematopoietic cells / Qigui Yu, Ningjie Hu, and Mario Ostrowski -- 23. Reverse genetics of nipah virus to probe viral pathogenicity / Misako Yoneda ... [et al.] -- 24. Baculovirus-mediated gene transfer into mesenchymal stem cells / Chien-Tai Tsai, Ching-Kuang Chuang, and Yu-Chen Hu.