Biological monitoring : prospects in occupational and environmental medicine

Foreword . . . . . . . . . . . . . . . . . . . . . . IX 1 The Importance of Biological Monitoring 1.1 The Development and Importance of Biological Monitoring in the DFG and MAK Commission . . . . 1 Dietrich Henschler 2 Internal Exposure and Haemoglobin Adducts 2.1 Biological Monitoring in Occupational and Environmental Medicine – The Present State of the Art and Future Prospects . . 5 Jürgen Angerer 2.2 Metabolic Profiling – AWay of Better Understanding External and Internal Exposure to Organic Stubstances . . . . . . . . . . . . . . . 16 Albert W. Rettenmeier 2.3 Biological Monitoring of Arylamines and Nitroarenes . . . . . . . . . . . . . . . . . . . 24 Gabriele Sabbioni V Contents 3 DNA Adducts 3.1 Genetic Cancer Susceptibility and DNA Adducts: Studies in Smokers and Coke Oven Workers . . . . 35 Magarita Rojas, Kroum Alexandrov, Helmut Bartsch and Berthold Spiegelhalder 3.2 The Detection of DNA Adducts in Biological Monitoring . . . . . . . . . . . . . . 46 Werner K. Lutz and Martin G. Maisch 3.3 32P-Postlabelling HPLC Analysis of DNA Adducts in Breast Tissue . . . . . . . . . . . . . . . . . . 57 Wolfgang Pfau 3.4 Studies of 8-Hydroxy-2
-Deoxyguanosine: A Biomarker for Oxidative DNA Damage in vivo? . . 68 Boleslaw Marczynski, Jürgen Hölzer and Michael Wilhelm 4 Susceptibility 4.1 Improved Methods of Phenotyping and Effect Monitoring for Evaluating the Risk to the Individual, using GSTT1 as an Example . . . 78 Ernst Hallier 4.2 Genetic Polymorphisms of Sulfotransferases as Susceptibility Parameters . . . . . . . . . . . . 84 Hansruedi Glatt 4.3 Genotyping and Phenotyping, Using NAT2 as an Example . . . . . . . . . . . . . . . . . . . 96 Klaus Golka and Meinolf Blaszkewicz 4.4 New High-thoughput Technology in the Diagnostic Screening of Susceptibility Factors . 103 Ricarda Thier, Thomas Brüning and Yon Ko VI Contents 5 Cytogenetic Parameters 5.1 Biological Monitoring with Cytogenetic Methods . . . 110 Günter Obe, Helga Fender and Gisela Wolf 5.2 Examples of the Use of Three-colour Chromosome Painting in Cytogenetic Biomonitoring . . . . . . . . 121 Erich Gebhart, Irmgard Verdorfer and Susann Neubauer 5.3 The Comet Assay as a Biological Monitoring Test . . . 130 Günter Speit, Oliver Merk and Andreas Rothfuß 6 Immunology 6.1 Immunoglobulins as Markers of Long-term Exposure to Allergenic Substances . . . . . . . . . . . . . . . 140 Hans Drexler 6.2 Immunological Effects of Polymorphic Key Enzymes . 146 Jürgen Lewalter 7 Epidemiology 7.1 Evaluation of Exposure in Epidemiological Studies . . 169 Kurt Ulm 7.2 Possibilities and Limitations of the Molecular Epidemiology of Workplace Exposures . . . . . . . . 175 Kurt Straif 8 Summary . . . . . . . . . . . . . . . . . . . . . . 191 Jürgen Angerer and Helmut Greim 9 Authors . . . . . . . . . . . . . . . . . . . . . . . 199