RNA'Protein Interaction Protocols. Methods in Molecular Biology, Volume 118

Cover -- Preface -- Contents -- Contributors -- 1 Labeling and Purification of RNA Synthesized by In Vitro Transcription -- 2 Joining RNA Molecules with T4 DNA Ligase -- 3 RNA8211;Protein Crosslinking with Photoreactive Nucleotide Analogs -- 4 The Methylene Blue Mediated Photocrosslinking Method for Detection of Proteins that Interact with Double45;Stranded RNA -- 5 Probing RNA8211;Protein Interactions by Psoralen Photocrosslinking -- 6 Identification and Sequence Analysis of RNA8211;Protein Contact Sites by N45;Terminal Sequencing and MALDI45;MS -- 7 RNA Footprinting and Modification Interference Analysis -- 8 Oligonucleotide45;Targeted RNase H Protection Analysis of RNA8211;Protein Complexes -- 9 Nitrocellulose Filter Binding for Determination of Dissociation Constants -- 10 Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays -- 11 PACE Analysis of RNA8211;Peptide Interactions -- 12 Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance -- 13 An Escherichia coli45;Based Genetic Strategy for Characterizing RNA Binding Proteins -- 14 Screening RNA45;Binding Libraries Using a Bacterial Transcription Antitermination Assay -- 15 In Vitro Genetic Analysis of RNA45;Binding Proteins Using Phage Display -- 16 In Vitro Selection of Aptamers from RNA Libraries -- 17 Identification of Specific Protein8211;RNA Target Sites Using Libraries of Natural Sequences -- 18 Northwestern Screening of Expression Libraries -- 19 Screening Expression Libraries with Solution45;Based Assay -- 20 An Immunoprecipitation45;RNA58;rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes -- 21 Purification and Depletion of RNP Particles by Antisense Affinity Chromatography -- 22 Purification of U Small Nuclear Ribonucleoprotein Particles -- 23 Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes -- 24 Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing -- 25 Mammalian In Vitro Splicing Assays -- 26 Yeast Pre45;mRNA Splicing Extracts -- 27 Prespliceosome and Spliceosome Isolation and Analysis -- 28 A Yeast Spliceosome Assay -- 29 Defining Pre45;mRNA cis Elements that Regulate Cell45;Specific Splicing -- 30 In Vivo SELEX in Vertebrate Cells -- 31 Purification of SR Protein Splicing Factors -- 32 Processing mRNA 339; Ends In Vitro -- 33 Analysis of Poly40;A41; Tail Lengths by PCR58; The PAT Assay -- 34 In Vitro Translation Extracts from Tissue Culture Cells -- 35 Messenger RNA Turnover in Cell45;Free Extracts from Higher Eukaryotes -- Index -- Last Page