Basic Cell Culture Protocols

Cover -- Preface -- Contents -- Contributors -- Basic Cell Culture -- Establishment44;Maintenance44; and Cloning of Human Dermal Fibroblasts -- Aging of Cultured Human Skin Fibroblasts -- Ex Vivo Maintenance of Differentiated Mammalian Cells -- Scale45;Up of Suspension and Anchorage45;Dependent Animal Cells -- Hollow45;Fiber Cell Culture -- Separation and Maintenance of Primary T45; and B45;Lymphocytes -- Human Pilosebaceous Culture -- Keratinocyte Culture -- Tissue Culture of Skeletal Muscle -- Isolation of Rat Liver Hepatocytes -- Primary Kidney Cells -- Human Thyroid Ephithelial Cells -- Derivation and Maintenance of Embryonic Stem Cell Cultures -- Culture of Cells From Human Tumors of the Nervous System on an Extracellular Matrix Derived from Bovine Corneal Endothelial C -- Culturing Primitive Hemopoietic Cells -- Collagen Gel Culture of the Human Hematopoietic Progenitors CFU45;GM44; CFU45;E44; and BFU45;E -- Long45;Term B45;Lymphoid Cultures from Murine Bone Marrow Establsihment and Cloning by Using Stromal Cell Line AC 646;21 -- Human Long45;Term Bone Marrow Culture -- In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells -- High Proliferative Potential Colony45;Forming Cells -- Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differntiation Factors -- Flow Sorting for Isolating CFU45;E -- Murine Bone Marrow45;Derived Macrophages -- Routine Testing of Cell Cultures and Their Products for Mycoplasma Contamination -- Fluorescent In Situ Hybridization 40;FISH41; for DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle -- Cytogenetic Techniques for Human Leukemias -- Visualization of Cell Replication Using Antibody to Proliferating Cell Nuclear Antigen -- Proliferation of Murine Myoblasts as Measured by Bromodeoxyuridine Incorporation -- Analysis of Cell Proliferation Using the Bromodeoxyuridine 47;Hoechst45;Ethidium Bromide Method -- mRNA In Situ Hybridization to In Vitro Cultured Cells -- Transmission and Scanning Electron Microsocope Preparation of Whole Cultured Cells -- Adhesion Molecules on Isolated and Cultured Microvascular Endothelial Cells Demonstrated by Immunofluorescence and Immune Ele -- Production of Heterologous Proteins Using the Baculovirus47;Insect Expression System -- Transfection and Transformation of Human Thyroid Epithelial Cells -- Analyzing Chemotaxis Using the Dunn Direct45;Viewing Chamber -- Computer45;Assisted Analysis of Single45;Cell Behavior -- Appendix -- Index -- Last Page