Protection of Endothelial Cells, Inhibition of Neointimal Hyperplasia by β-elemene in an Injured Artery
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Lingyan Wu , Guixue Wang , Shutang Tang , Guang Long , Tieying Yin
- چاپ و سال / کشور: 2011
Description
Aims It is generally accepted that the oxidative stress and the proliferative activity of vascular smooth muscle cells (VSMCs) contribute to the pathogenesis of neointimal hyperplasia after vascular injury. Although â-elemene (â- 1-methyl-1-vinyl-2, 4-diisopropenyl-cyclohexane) has been used as an antitumour drug, its therapeutic effect on vascular diseases has not yet been determined. In this study, we investigated whether â-elemene could inhibit oxidative damage of vascular endothelial cells, suppress VSMCs growth and prevent neointimal hyperplasia. Methods and results â-elemene can increase the survival rate of human umbilical vein endothelial cells in vitro. By measuring the malondialdehyde content, total antioxidant capacity, superoxide dismutase activity, catalase activity, glutathione peroxidase activity and nitric oxide levels, we assessed the protective effect of â-elemene in the vascular endotheliummodel against oxidant-induced injury.جoreover, â-elemene inhibited cell proliferation and induced apoptosis in cultured VSMCs. In a flow culture system, â-elemene reduced the migration distance of VSMCs. By transwell migration assay, â-elemene was found to reduce themigration cell number of VSMCs, but not affect the HUVECsmigration. In a rat carotid artery balloon injury model, administration of â-elemene significantly reduced the ratio of intimal area to medial area and neointima formation. Conclusions Our results indicate that â-elemene is effective in protecting the endothelial cells from injury induced by hydrogen peroxide in vitro, inhibiting smooth muscle cell proliferation/migration and inhibiting neointima formation in vivo after vascular injury.
Cardiovasc Drugs Ther (2011) 25:233–242 Published online: 24 May 2011
