Epitope analysis of GAD65 autoantibodies in adult-onset type 1 diabetes and latent autoimmune diabetes in adults with thyroid autoimmunity
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ping Jin , Gan Huang , Jian Lin ,Shuoming Luo , Zhiguang Zhou
- چاپ و سال / کشور: 2011
Description
the spontaneous antibodies recognized and whether the epitope-specific GAD65Abs could be associated with the development of thyroid autoimmunity in Chinese adult-onset type 1 diabetes (T1DM) and latent autoimmune diabetes in adults (LADA). The levels of GAD65Abs and their reactivities to N-terminal (GAD65-N), middle (GAD65-M) and C-terminal (GAD65-C) regions of human GAD65 were measured by radioligand assay in 109 patients with adult-onset T1DM and 107 with LADA. TPOAb, TGAb and the genotypes of HLADQA1-DQB1 were determined. The percentage of LADA patients with GAD65-NAb was significantly higher than that of adultonset T1DM patients (21.5% vs. 11.0%, P = 0.037), but LADA patients with GAD65-CAb less than T1DM patients (47.7% vs. 70.6%, P = 0.001). LADA patients with both GAD65-M and GAD65-CAb (GAD65-M ? CAb) appeared to be at higher risk for the development of thyroid autoimmunity, lower serum C-peptide level and the requirement for insulin therapy (P\0.05). More frequent T1DM patients with HLADQA1*03-DQB1*0303 developed GAD65-M ? CAb (55.8% vs. 35.1%, P = 0.008). In comparison with those without thyroid autoimmunity, more frequent T1DM patients and LADA patients with thyroid autoimmunity displayed GAD65-M ? CAbs (44.0% vs.16.9% and 53.1% vs. 17.3%, P = 0.002 and \0.001, respectively) with a diagnostic specificity of 83.1 or 82.7% for thyroid autoimmunity, respectively. LADA patients with GAD65-M ? CAbs had clinical features similar to T1DM patients. Adult-onset T1DM and LADA patients with GAD65-M ? CAbs are at an increased risk for the development of thyroid autoimmunity.
Acta Diabetol (2011) 48:149–155 DOI 10.1007/s00592-010-0250-0 Received: 22 October 2010 / Accepted: 20 December 2010 / Published online: 7 January 2011 Springer-Verlag 2011
