Modulation of tissue factor and thrombomodulin expression in human aortic endothelial cells incubated with high glucose
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Huang-Joe Wang Huey-Chun Huang Yi-Ching Chuang Pei-Ju Liao Den-Mei Yang Wen K. Yang Haimei Huang
- چاپ و سال / کشور: 2010
Description
Diabetes is often associated with atherothrombosis. It is unknown whether high glucose can modulate the expression of tissue factor (TF) and thrombomodulin (TM) in human aortic endothelial cells (HAECs). HAECs were treated with a lower-degree high glucose condition (LG, 11.2 mM) for 8 days and a higher-degree high glucose condition (HG, 30 mM) for 4–6 h. Methoxyphenyl tetrazolium inner salt assay, real-time polymerase chain reaction, western blot, and TF activity assay were performed. In HAECs, both LG and HG conditions were nontoxic. LG caused a 74 ± 20% decrease (P = 0.273) and HG caused a 57 ± 5% decrease in TF mRNA expression (P = 0.001). LG caused a 53 ± 13% decrease (P = 0.036) and HG caused a 75 ± 10% decrease in TF protein expression (P = 0.096). TF activity was not significantly changed by LG (127 ± 13%, P = 0.40) or HG treatments (120 ± 42%, P = 0.70). In contrast, LG caused a 153 ± 16% increase (P = 0.03) and HG caused a 211 ± 20% increase in TM mRNA expression (P = 0.005). LG caused a 131 ± 31% increase (P = 0.35) and HG caused a 140 ± 9% increase in TM protein expression (P = 0.006). Different high glucose conditions do not provide the sufficient stress required to induce TF expression in HAECs. In contrast, high glucose conditions can induce TM expression in HAECs
Acta Diabetol DOI 10.1007/s00592-010-0182-8 Received: 6 October 2009 / Accepted: 24 February 2010 Springer-Verlag 2010
