Concurrent p16 methylation pattern as an adverse prognostic factor in multiple myeloma: a methylation-specific polymerase chain reaction study using two different primer sets
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Geon Park & Seong Ho Kang & Jae Hoon Lee & Cheolwon Suh & Miyoung Kim & Seung Man Park & Tae Young Kim & Bora Oh & Hyun Jung Min & Sung Soo Yoon & In
- چاپ و سال / کشور: 2010
Description
Disruption of cell cycle control genes, including p16, is known to contribute to the cancerogenesis of multiple myeloma (MM). We investigated the methylation status of p16 and its association with common cytogenetic changes, clinicolaboratory findings, and survival in MM. Methylation-specific polymerase chain reaction was performed in 99 newly diagnosed MM patients using two different sets of primers (p16M1 and p16M2). Four patterns of p16 promoter methylation were observed: (1) concurrent methylation of p16M1 and p16M2 (P1P2), 27.3%; (2) methylation of p16M1 alone (P1N2), 7.1%; (3) methylation of p16M2 alone (N1P2), 26.3%; and (4) no methylation
Received: 2 March 2010 / Accepted: 2 August 2010 / Published online: 19 August 2010
