Functional analysis and gene expression profile of umbilical cord blood regulatory Tcells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Giovanni Fernando Torelli & Roberta Maggio & Nadia Peragine & Sabina Chiaretti & Maria Stefania De Propris & Barbarella Lucarelli & Maria Screnci & Ma
- چاپ و سال / کشور: 2011
Description
The aim of the study was to analyze and compare the functional properties and the gene expression profile of regulatory Tcells (Tregs) isolated from cord blood (CB) units (n=23) and from the peripheral blood (PB) of adult normal donors (n=13). Tregs were purified from mononuclear cells and expanded for 6 days with anti-CD3, anti-CD28, and IL- 2. CB and PB Tregs presented similar immunophenotypic features. However, Tregs isolated from CB presented a much higher expansion capacity; this was confirmed by the genomic characterization that showed in CB-derived Tregs significant enrichments of genes involved in cell proliferation, chromatin modification, and regulation of gene expression. All samples were positive for the FoxP3 gene and protein after expansion. CB and PB expanded Tregs exerted a comparable and potent suppressive function on the proliferative reaction of autologous T cells stimulated by allogeneic dendritic cells and presented a high in vitro IL-10 production capacity. Gene profile analysis also revealed for PB Tregs significant enrichments of genes involved in the adaptive immune response. These data offer further insights into the understanding of the biology of CB transplantation indicating a possible role played by CB Tregs in the suppression of the allogeneic T cell response.
Ann Hematol DOI 10.1007/s00277-011-1288-y Received: 20 February 2011 / Accepted: 20 June 2011
