CD14-/CD34+ is the founding population of umbilical cord blood-derived endothelial progenitor cells and angiogenin1 is an important factor promoting the colony formation
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Sakchai Sudchada & Pakpoom Kheolamai & Yaowalak U-Pratya & Methichit Chayosumrit & Aungkura Supokawej & Sirikul Manochantr & Chairat Tantrawatpan & Ha
- چاپ و سال / کشور: 2011
Description
The origin of endothelial progenitor cells (EPCs) in umbilical cord blood (UCB) is unknown. In this study, we explored the origin of UCB-derived EPCs by culturing CD14+ or CD14− subpopulation separately and co-culturing these two subpopulations either with or without transwells. We found no colony formation with CD14+ or CD14− subpopulation alone, but there were EPC colonies observed in direct co-cultures of both subpopulations. Transwell culture system was used to further study the effect of cytokines on EPC colony formation. We observed the presence of EPC colonies derived from CD14− subpopulation in the presence of CD14+ subpopulation in the upper compartment whereas there was no colony generated from CD14+ subpopulation with CD14− subpopulation in the upper compartment. Therefore, CD14− subpopulation is likely to be the origin of EPCs and EPC colony derivation requires cytokines released from CD14+ subpopulation. We further characterized the founding population of UCB-derived EPCs by separating CD14− subpopulation into CD14−/CD34+ and CD14−/CD34− subpopulations. There were colonies observed only in co-cultures of CD14+ with CD14−/CD34+ subpopulation but not with CD14−/CD34− subpopulation either with or without transwells. We screened 42 cytokines involving in angiogenesis using an ELISA array in the supernatant collected from CD14+ compared to CD14− subpopulations. We found consistently the presence of angiogenin1 in the supernatant of CD14+ subpopulation but not in that of CD14− subpopulation. The addition of angiogenin1 in culture of CD14− subpopulation yielded EPC colonies. We conclude that UCB-derived EPCs are confined to CD14−/ CD34+ subpopulation and angiogenin1 released from CD14+ subpopulation may be an important factor promoting the EPC colony formation.
Ann Hematol DOI 10.1007/s00277-011-1303-3 Received: 14 April 2011 / Accepted: 20 July 2011
