Immunoconjugated gold nanoshell-mediated photothermal ablation of trastuzumab-resistant breast cancer cells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Laura B. Carpin Lissett R. Bickford Germaine Agollah Tse-Kuan Yu Rachel Schiff Yi Li Rebekah A. Drezek
- چاپ و سال / کشور: 2010
Description
Trastuzumab is a FDA-approved drug that has shown clinical efficacy against HER2? breast cancers and is commonly used in combination with other chemotherapeutics. However, many patients are innately resistant to trastuzumab, or will develop resistance during treatment. Alternative treatments are needed for trastuzumab-resistant patients. Here, we investigate gold nanoparticle-mediated photothermal therapies as a potential alternative treatment for chemotherapy-resistant cancers. Gold nanoshell photothermal therapy destroys the tumor cells using heat, a physical mechanism, which is able to overcome the cellular adaptations that bestow trastuzumab resistance. By adding anti-HER2 to the gold surface of the nanoshells as a targeting modality, we increase the specificity of the nanoshells for HER2? breast cancer. Silica–gold nanoshells conjugated with anti-HER2 were incubated with both trastuzumab-sensitive and trastuzumab-resistant breast cancer cells. Nanoshell binding was confirmed using twophoton laser scanning microscopy, and the cells were then ablated using a near-infrared laser. We demonstrate the successful targeting and ablation of trastuzumab-resistant cells using anti-HER2-conjugated silica–gold nanoshells and a near-infrared laser. This study suggests potential for applying gold nanoshell-mediated therapy to trastuzumabresistant breast cancers in vivo.
Breast Cancer Res Treat (2011) 125:27–34 Received: 4 February 2010 / Accepted: 13 February 2010 / Published online: 10 March 2010 Springer Science+Business Media, LLC. 2010
