Lipopolysaccharide (LPS) of Helicobacter modulates cellular DNA repair systems in intestinal cells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Pasqua Cavallo Antonia Cianciulli Vincenzo Mitolo Maria Antonietta Panaro
- چاپ و سال / کشور: 2010
Description
The epithelium of the intestinal tract is exposed to a variety of genotoxic agents, both exogenous and endogenous, that can injure nuclear and mitochondrial DNA. DNA damage can be repaired by a series of DNA repair enzymes, while defects in this system will make these cells once more susceptible to malignant transformation or cell death. Recent studies suggest that intestinal bacteria may contribute to induce inflammation in individuals afflicted by inflammatory bowel disease (IBD), increasing the risk of developing colon cancer. Accumulating evidence suggests that Helicobacter organisms are linked to IBD as well as to gastric and colon cancer. Therefore, the focus of this study was to evaluate the effect of lipopolysaccharide (LPS) isolated from Helicobacter on modulating the DNA repair system. We used an in vitro model represented by two colon carcinoma cell lines, the DNA repair-proficient SW480 and the DNA repair-deficient LoVo, and transfected with a UVC-irradiated psVbeta- galactosidase plasmid. We observed that LPS, by upregulating the expression of inducible nitric oxide (NO), leads to an increased NO release, demonstrating that LPS is able to interfere with the DNA repair machinery of intestinal cells, thus increasing the risk of permanent genotoxic effects.
Clin Exp Med (2011) 11:171–179 DOI 10.1007/s10238-010-0118- Received: 1 July 2010 / Accepted: 18 October 2010 / Published online: 11 November 2010
