Gene expression of O-GlcNAc cycling enzymes in human breast cancers
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Anna Krzes´lak Ewa Forma Magdalena Bernaciak Hanna Romanowicz Magdalena Brys
- چاپ و سال / کشور: 2011
Description
O-GlcNAcylation is an abundant, dynamic, and inducible posttranslational modification in which single b-N-acetylglucosamine residues are attached by Oglycosidic linkage to serine or treonine residues. It is suggested that abnormally regulated O-GlcNAcylation may contribute to the pathology of cancer. Cycling of OGlcNAc residues on intracellular proteins is controlled by two enzymes, O-GlcNAc transferease (OGT), which catalyses the addition of O-GlcNAc residues and nucleocytoplasmic b-N-acetylglucosaminidase (O-GlcNAcase; encoded by MGEA5 gene), an enzyme involved in the removal of O-GlcNAc. In this study, relationship between the mRNA expressions of genes coding O-GlcNAc cycling enzymes in breast ductal carcinomas and clinicopathological parameters were analyzed. The results showed that poorly differentiated tumors (grade II and III) had significantly higher OGT expression than grade I tumors. Contrary, MGEA5 transcript levels were significantly lower in grade II and III in comparison with grade I tumors. The Spearman rank correlation showed the expressions of OGT and MGEA5 in breast cancer was negatively correlated (r = -0.430, P = 0.0002). Lymph node metastasis status was significantly associated with decreased MGEA5 mRNA expression. This result suggests that elevation in O-GlcNAc modification of proteins may be implicated in breast tumor progression and metastasis.
Clin Exp Med DOI 10.1007/s10238-011-0138-5 Received: 19 January 2011 / Accepted: 21 April 2011
