Study on the Expression and Signifi cance of TGF-β1, p-ERK1/2and K-ras in Colorectal Cancer Using Tissue Microarray Technique
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Xin HU1 Yu-ting KUANG2 Mao-min SUN3 Ying-ying WANG1 Yu-juan ZHANG1 Ling-ling GUO1 Shou-li WANG1
- چاپ و سال / کشور: 2011
Description
This study aimed to explore the expression and significance of transforming growth factor β1(TGF-β1), extracellular signal-regulated kinases 1/2 (ERK1/2), and K-ras in colorectal cancer (CRC) using tissue microarray technology. METHODS The expressions of TGF-β1, ERK1/2, and K-ras in colon cancer cells taken from the specimens of 92 CRC patients (stage I: 16 cases, stage II: 28 cases, stage III: 24 cases, and stage IV: 24 cases) were analyzed using tissue microarray technology and immunohistochemistry, and compared with those of 20 normal colon tissue samples. RESULTS High immunoreactive scores (IRS) of TGF-β1, p-ERK1/2, and K-ras protein in CRC were obtained, which were 66.3% (61/92), 59.8% (55/92), and 48.9% (45/92), respectively, and those in normal epithelial cells of colon were 10% (2/20), 20% (4/20), and 30% (6/20), respectively (P < 0.05). The expressions of TGF-β1 and ERK1/2 in CRC at stage I were 37.5% and 31.3%, respectively, and those in CRC at stage IV were 83.3% and 79.3%, respectively, with statistically significant differences. No signifi cant relationship was found between K-ras expression and tumor stages (P > 0.05). CONCLUSION High level expressions of TGF-β1 and ERK1/2 are closely related to the clinical stages of colon cancer and crosstalk may exist between the 2 signal pathways.
Clin Oncol Cancer Res (2011) 8: 21-26 DOI 10.1007/s11805-011-0554-z
