Effects of a composition containing lactoferrin and lactoperoxidase on oral malodor and salivary bacteria:a randomized, double-blind, crossover, placebo-controlled clinical trial
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Kouichirou Shin & Ken Yaegaki ,Takatoshi Murata ,Hisataka Ii ,Tomoko Tanaka ,Izumi Aoyama ,Koji Yamauchi ,Tomohiro Toida ,Keiji Iwatsuki
- چاپ و سال / کشور: 2010
Description
We report a clinical trial of the effects of test tablets containing bovine lactoferrin and lactoperoxidase on oral malodor and salivary bacteria. Fifteen subjects with volatile sulfur compounds (VSCs) in mouth air above the olfactory threshold (H2S >1.5 or CH3SH >0.5 ng/10 ml) as detected by gas chromatography were enrolled in the trial. Either a test or a placebo tablet was ingested twice at 1-h intervals in two crossover phases. Mouth air was monitored for VSC levels at the baseline before ingestion of a tablet, 10 min after the first ingestion, 1 h (just before the second ingestion), and 2 h after the first ingestion. Whole saliva was analyzed at the baseline and at 2 h for bacterial numbers. At 10 min, the level of CH3SH was significantly lower in the test group (median [interquartile range]=0.28 [0.00–0.68]ng/10 ml) compared to that in the placebo group (0.73 [0.47–1.00]ng/10 ml; P=0.011). The median concentration of CH3SH in the test group was below the olfactory threshold after 10 min until 2 h, whereas the level in the placebo group was above the threshold during the experimental period. No difference in the numbers of salivary bacteria was detected by culturing or quantitative PCR, but terminal restriction fragment length polymorphism detected one fragment with a significantly lower copy number at 2 h in the test group (mean ± standard error, 4.89±0.11 log10 copies/10 ىl) compared to that in the placebo group (5.38±0.15 log10 copies/10 ىl; P=0.033). These results indicate a suppressive effect of the test composition on oral malodor and suggest an influence on oral bacteria.
Clin Oral Invest (2011) 15:485–493 DOI 10.1007/s00784-010-0422-x Received: 27 January 2010 / Accepted: 26 April 2010 / Published online: 29 May 2010
