Salivary shedding of Epstein–Barr virus and cytomegalovirus in people infected or not by human immunodeficiency virus 1
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Talita Ribeiro Tenَrio de França & Alessandra de Albuquerque Tavares Carvalho & Valder Barbosa Gomes & Luiz Alcino Gueiros & Stephen Ross Porter & Ja
- چاپ و سال / کشور: 2011
Description
The purpose of this study is to determine the frequency of EBV and CMV DNA detection in saliva of HIV infected and non-HIV individuals and their siblings. The study group comprised 240 individuals. Group 1 comprised of 40 HIV-infected patients, group 2 40 non- HIV individuals, group 3 two siblings for each patient from group 1 (n=80), and group 4 two siblings for each individual from group 2 (n=80). Non-stimulated whole saliva was collected, DNA was extracted, and amplification was performed using a nested PCR protocol. EBV and CMV DNA was detected in 7/40 (17.5%) and 5/40 (12.5%) individuals from group 1, 8/40 (20%) and 3/40 (7.5%) from group 2, 11/80 (13.8%) and 2/80 (2.5%) from group 3, and 8/80 (10%) and 1/80 (1.3%) from group 4, respectively. Five (71.4%) out of seven HIV/EBV coinfected individuals of group 1 had a relative also infected with EBV (OR=11.25, CI [1.75–72.5], p=0.011). Regarding group 2, among the eight non-HIV and EBV-infected individuals, three (37.5%) had a relative also positive to EBV (p=0.320). No individual HIV/CMV coinfected had a relative CMV infected (p=1.00). Also, only one non-HIV and CMVinfected individual had a relative also positive to CMV (p=0.075). EBV and CMV DNA was detected mainly in those who had HIV viral load counts <400/mL (71%, p=0.2 and 100%, p=1, respectively) and those who had CD4 T cells counts between 200 and 400/mm3 (57%, p=0.544 and 60%, p=0.249, respectively). HIV-infected individuals and healthy controls showed a similar frequency of viral DNA detection. EBV DNA was significantly amplified in saliva of household members of HIV/EBV coinfected individuals
Clin Oral Invest DOI 10.1007/s00784-011-0548-5 Received: 3 June 2010 / Accepted: 14 March 2011
