Cytotoxicity of denture adhesives
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Pedro Sousa de Gomes & Maria Helena Figueiral & Maria Helena R. Fernandes & Crispian Scully
- چاپ و سال / کشور: 2010
Description
Ten commercially available denture adhesives, nine soluble formulations (six creams, three powders) and one insoluble product (pad), were analyzed regarding the cytotoxicity profile in direct and indirect assays using L929 fibroblast cells. In the direct assay, fibroblasts were seeded over the surface of a thick adhesive gel (5%, creams; 2.5%, powders and pad). In the indirect assay, cells were cultured in the presence of adhesive extracts prepared in static and dynamic conditions (0.5–2%, creams; 0.25–1%, powders and pad). Cell toxicity was assessed for cell viability/proliferation (MTT assay) and cell morphology (observation of the F-actin cytoskeleton organization by confocal laser scanning microscopy). Direct contact of the L929 fibroblasts with the thick adhesive gels caused no, or only a slight, decrease in cell viability/proliferation. The adhesive extracts (especially those prepared in dynamic conditions) caused significantly higher growth inhibition of fibroblasts and, in addition, caused doseand time-dependent effects, throughout the 6–72 h exposure time. Also, dose-dependent effects on cell morphology, with evident disruption of the F-actin cytoskeleton organization, were seen in the presence of most adhesives. In conclusion, the adhesives possessed different degrees of cytotoxicity, but similar dose- and time-dependent biological profiles.
Clin Oral Invest DOI 10.1007/s00784-010-0464-0 Received: 26 April 2010 / Accepted: 25 August 2010
