Nafamostat mesilate, a potent tryptase inhibitor, modulates periodontitis in rats
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Marinella Holzhausen & Rodrigo D. P. Balejo &Guilherme M. Lara & Sheila C. Cortelli & Wilson A. Saad & José R. Cortelli
- چاپ و سال / کشور: 2010
Description
Previous reports have demonstrated increased tryptase-like proteolytic activity in the crevicular fluid of patients with periodontal disease. In the present study, we have investigated the effect of tryptase inhibition with nafamostat mesilate (NM, 6-amino-2-naphtlyl p-guanidinobenzoate dimethansulfonate) on the development of experimental periodontitis in rats. Eighty (80) male Wistar rats were randomly separated into four groups: Control group, NM group (daily 0.1 mg/kg body weight of NM, i.p.), Ligature group (ligature placed at lower right first molars), and NM+Ligature group. The amount of alveolar bone loss (ABL) around the mesial root surface of the first mandibulary molar, as well as the myeloperoxidase (MPO) activity, and total proteolytic activity [N-benzoyl-L-arginine-p-nitroanilide (BApNA) substrate] were determined at 7 and 14 days. NM led to significantly (p<0.05) decreased ABL in animals subjected to ligatureinduced periodontitis. Tryptase inhibition prevented the onset of significant ABL at 7 days of experiment (0.44±0.16 and 0.60±0.22, p>0.05, NM+Ligature and Control, respectively) and significantly decreased the ABL at 14 days (0.97±0.17 versus 1.82±0.26, p<0.001, NM+Ligature versus Ligature, respectively). In addition, NM significantly decreased MPO and total proteolytic activity at 14 days (p<0.05). These data provided evidence that tryptase inhibition with NM attenuates gingival granulocyte infiltration and ABL in an experimental model of periodontitis in rats.
Clin Oral Invest DOI 10.1007/s00784-010-0463-1 Received: 22 April 2010 / Accepted: 25 August 2010
