Early outgrowth EPCs generation is reduced in patients with Buerger’s disease
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Yoshio Katsuki Ken-ichiro Sasaki Yasuyuki Toyama Masanori Ohtsuka Hiroshi Koiwaya Takaharu Nakayoshi Tsutomu Imaizumi
- چاپ و سال / کشور: 2010
Description
Background Buerger’s disease often shows poor collateral artery generation (i.e. neovascularization) in the ischemic limbs. However, the etiology has not yet been clarified. Circulating endothelial progenitor cells (EPCs) derived from bone marrow contribute to neovascularization in the multi-step process which includes the following capacities; mobilization, differentiation, adhesion, migration, invasion and secretion. Materials and methods We assessed EPCs capacities in vitro and ex vivo in age- and sex-matched controls (n = 12) and patients with Buerger’s disease (n = 12), derived from peripheral blood-derived mononuclear cells (PB-MNCs). Results In the flow cytometry analysis, the numbers of circulating EPC (CD34?/KDR? or CD133?/KDR? PBMNC) were similar between controls and patients with Buerger’s disease. Next, we cultured PB-MNC to obtain EPCs. The number of early outgrowth EPCs was significantly decreased in patients with Buerger’s disease (p\0.005), indicating the reduced generation of early outgrowth EPCs in Buerger’s disease. However, adhesion, migration, invasion and secretion capacities were not impaired in patients with Buerger’s disease. Conclusions The early outgrowth EPCs generation is reduced in patients with Buerger’s disease
Clin Res Cardiol (2011) 100:21–27 DOI 10.1007/s00392-010-0198-7 Received: 24 March 2010 / Accepted: 24 June 2010 / Published online: 4 July 2010
