The effect of post-mortem delay on immunohistochemical labelling—a short review
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Cheryl L. Scudamore & Heather K. Hodgson & Lee Patterson & Anna Macdonald & Fiona Brown & Ken C. Smith
- چاپ و سال / کشور: 2010
Description
Immunohistochemistry (IHC) is a routine technique in diagnostic and experimental pathology. Most protocols are developed for tissues which have been optimally collected into fixative or frozen rapidly after death. In practice, tissue samples often come from animals that have died unexpectedly during an experimental study or from diagnostic samples where there is a significant delay before fixation. Continued cellular metabolism and enzyme activity (autolysis) after death have the potential to alter the expression of antigens either positively through initial cellular responses to anoxia or negatively by degradation of tissue proteins. It is important to understand the effect that these changes may have on the reliability of the chosen tissue markers for both diagnostic and quantitative analysis. This paper will review the literature in this area and illustrate the effect of temperature and time of post-mortem delay in rat and dog tissues for some commonly used cellular markers: cytokeratin, vimentin, factor VIII-related antigen, Ki67, PCNA and activated caspase 3 using previously unpublished data from a number of studies.
Comp Clin Pathol (2011) 20:95–101 DOI 10.1007/s00580-010-1149-4 Received: 20 September 2010 / Accepted: 7 December 2010 / Published online: 24 December 2010
