Comparative study on determination of plasma thyroid hormones by chemiluminescence and electrochemiluminescence immunoassay methods in sheep
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Behrad Eshratkhah & Hamid Rajabian & Davar Namvar & Salar Eshratkhah & Salar Mohammadi Bastam
- چاپ و سال / کشور: 2010
Description
The aim of this study was to compare plasma thyroid hormone concentrations by both chemiluminescence (CLIA) and electrochemiluminescence immunoassay (ECLIA) methods in sheep. Blood samples were taken from the jugular vein of 25 clinically healthy, non-pregnant adult ewes. The plasma was analyzed to determine thyroxine (T4), tri-iodothyronine (T3), free thyroxine (fT4) and free tri-iodothyronine (fT3) concentrations. The data from this study indicates, there were significant differences in the T4 (P<0.0001), T3(P<0.01) and fT3(P<0.01) concentrations between the two methods, and the levels of these hormones were higher when using the ECLIA method. In determining thyroid hormones using the CLIA and ECLIA methods, significant positive correlations were found between T4 and fT4 (P<0.001, r=0.703; P<0.0001, r=0.806) also between T3 and fT3 (P<0.0001, r=0.922; P<0.0001, r=0.923) concentrations, respectively. The linear regression analysis of these hormones showed that the CLIA and ECLIA results were significantly correlated (T4 (P<0.01, r=0.583), T3 (P<0.0001, r=0.898), fT4 (P<0.0001, r=0.796) and fT3 (P<0.0001, r=0.898).
Comp Clin Pathol (2011) 20:135–138 DOI 10.1007/s00580-010-0967-8 Received: 19 September 2009 / Accepted: 7 January 2010 / Published online: 13 February 2010
