Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Anne Mathers & Gareth O. Evans & John Bleby
- چاپ و سال / کشور: 2010
Description
Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBCY) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias.
Comp Clin Pathol DOI 10.1007/s00580-010-1147-6 Received: 15 September 2010 / Accepted: 7 December 2010
