Seasonal variation in antioxidant enzyme activity in seminal plasma in Holstein bulls
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Reza Asadpour & Hossein Tayefi-Nasrabadi
- چاپ و سال / کشور: 2010
Description
Seminal oxidative stress status is emerging as a significant prognostic tool in assisted reproductive technology. A dynamic interplay between pro- and antiantioxidant substances in the ejaculate is essential. In this study, we determined seasonal changes in the activity of the antioxidant enzyme defence system comprising catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in seminal plasma (SP) of mature Holstein bull’s semen. Samples were collected by a bovine artificial vagina from 30 sexually mature Holstein bulls, and CAT, GPx and SOD activities were determined for different seasons. The CAT enzymatic activity determined in SP was greater in autumn as compared with other seasons (P<0.01). Significantly lower GPx activity was found in winter compared with other seasons (62±12 versus 133±35; P<0.01). However, SOD was not significantly affected by the season. The results reported confirm the influence of season on sperm quality. Furthermore, this study presents convincing evidence that SP composition is important in determining the antioxidant defence system of ejaculated spermatozoa.
Comp Clin Pathol DOI 10.1007/s00580-010-1080-8 Received: 4 May 2010 / Accepted: 25 August 2010
