Immuno-histochemical staining expression of Ki67 protein in canine mammary gland carcinoma
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Annahita Rezaie & Abbas Tavasoli & Mitra Mehrazma & Alireza Bahonar
- چاپ و سال / کشور: 2010
Description
Canine mammary gland carcinoma is ranked second after skin tumours in dogs in terms of frequency. Due to certain microscopic and biological features, canine tumours are very similar to human carcinomas, which may make the dog a good animal model. The objective of this study was to describe Ki67 protein expression properties using immuno-histochemical (IHC) staining using human antibody. In this study, 30 mammary gland tumour samples were studied. The histological diagnosis was made on the basis of the current World Health Organization classification for canine mammary tumours. Tumours were then graded histologically in accordance with the Elston and Ellis method for human breast tumours. IHC staining using MIB-1 antibody for detecting expression of Ki67 proteins (pKi67), was performed on canine mammary gland carcinoma tissue. Results from the semi-quantitative IHC assessment of pKi67 showed that 20% were negative, 20% were one plus, 36.5% were two plus, 23.3% were three plus. There was a significant difference between histological grade and pKi67 expression. This method would help the pathologist and clinician in determining a more accurate prognosis and treatment in canine mammary carcinoma and facilitate comparative studies of canine and human research.
Comp Clin Pathol DOI 10.1007/s00580-010-1032-3 Received: 31 January 2010 / Accepted: 26 May 2010
