Characterization of the siderophore transport genes in Aeromonas salmonicida subsp. salmonicida
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Mohsen Najimi
- چاپ و سال / کشور: 2011
Description
Bacteria have developed multiple mechanisms to obtain iron from the host. Aeromonas salmonicida subsp. salmonicida produces a catechol-type siderophore under iron starvation conditions. In this study, we characterized six ABC transporter proteins immediately downstream of a sigma factor as AsbI, which were denominated AsbH, AsbJ, AsbK, AsbL, AsbQ, and AsbM, and a siderophore receptor as AsbE. All studied genes showed a high similarity with other siderophore transporter genes in other gram-negative bacteria. Interestingly, asbH and asbJ genes were shown to encode a mixed-permease, ATP-binding protein. Reverse transcriptase PCR analysis demonstrated that the gene cluster is arranged into two transcriptional units: (1) asbIHJKLQM and (2) asbE. It also showed that the expression of these genes is regulated by iron concentration
Comp Clin Pathol DOI 10.1007/s00580-011-1310-8 Received: 7 March 2011 / Accepted: 18 July 2011
