Beneficial Effects of Follistatin in Hepatic Ischemia-Reperfusion Injuries in Rats
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Mami Kanamoto Mitsuo Shimada Yuji Morine Tomoharu Yoshizumi Satoru Imura Toru Ikegami Hiroki Mori Yusuke Arakawa
- چاپ و سال / کشور: 2010
Description
Background Ischemia-reperfusion injury has been demonstrated in a variety of clinical settings. The morbidity associated with liver transplantation and major hepatic resections is partly a result of ischemia-reperfusion injury. Follistatin, an activin-binding protein, binds to activins and subsequently blocks their action. It was reported that blockade of the action of activin with administration of follistatin accelerates recovery from ischemia renal injury. This study was conducted to investigate the involvement of the activin–follistatin system in hepatic ischemiareperfusion injury. Methods Total hepatic ischemia for 30 min was performed followed by reperfusion in a rat model. Rats were divided into two groups: a follistatin group and a control group. Follistatin (1 lg/body), which is an activin-binding protein, was administered at the time of reperfusion. Results Though 80% of animals survived in the follistatin group, four of five animals died in the control group within 3 days after reperfusion (p\0.05). AST was significantly lower at 3 h after reperfusion in the follistatin group (p\0.05). LDH was also lower at 6 h after reperfusion in the follistatin group (p\0.05). Follistatin inhibited the mRNA expression of the bA subunit of activin. Moreover, the expression of IL-6, which is an inflammatory cytokine, was suppressed at 6 h after reperfusion in the follistatin group (p\0.05). Conclusions The present study demonstrated that treatment with follistatin reduced the expression of IL-6 and activin resulting in beneficial support for hepatic ischemiareperfusion injuries
Dig Dis Sci (2011) 56:1075–1081 DOI 10.1007/s10620-010-1401-4 Received: 12 April 2010 / Accepted: 12 August 2010 / Published online: 8 September 2010
