Transient Stasis of Pancreatic Fluid Flow Together with Mild Injury of the Pancreatic Duct Cause Chronic Pancreatitis
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Taizo Yamaguchi Yasuyuki Kihara Mitsuyoshi Yamamoto Makoto Otsuki
- چاپ و سال / کشور: 2011
Description
Background Little is known about the etiopathogenesis of chronic pancreatitis, due mainly to the lack of simple animal models suitable to study inflammatory and fibrogenetic processes in the pancreas. Aims The purpose of this study was to examine whether transient congestion of pancreatic fluid flow alone or slight ductal injury alone is sufficient, or where both are required, to induce chronic pancreatic injury. Methods Three different models of pancreatitis were tested in rats induced by retrograde intraductal infusion of 40 ll/100 g body weight of 0.01% agarose (group A), 40 ll/100 g body weight of 0.1% sodium taurocholate (group T), or a mixture of the two solutions (group M). Histological alterations of the pancreas were examined by hematoxylin-eosin staining, changes in type IV collagen structure were studied by immunostaining, and the gelatinolytic activity of latent and active matrix metalloproteinase- 2 (MMP-2) was analyzed by zymography. Results In group A and T rats, histological alterations of the pancreas and the gelatinolytic activity of MMP-2 returned to baseline levels by day 14, and immunoreactivity for type IV collagen appeared as continuous lines along the basement membrane. In group M rats, however, acinar damage, fibrosis and fatty degeneration were observed even on day 56, and type IV collagen was detected as discontinuous lines until day 56. MMP-2 was significantly elevated from day 5 to day 42. Conclusions Co-existence of transient stasis of pancreatic fluid flow, together with mild damage to the pancreatic duct and acinar cells, exert synergistic effects on the development of persistent pancreatic injury with continuous disorganization of type IV collagen in the basement membrane of the ducts.
Dig Dis Sci (2011) 56:2326–2332 DOI 10.1007/s10620-011-1586-1 Received: 10 September 2010 / Accepted: 14 January 2011 / Published online: 28 January 2011
