Dynamics of Non-conventional Intraepithelial Lymphocytes—NK, NKT, and cd T—in Celiac Disease: Relationship with Age, Diet, and Histopathology
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Sara Calleja Santiago Vivas Marı´a Santiuste Laura Arias Mercedes Hernando Esther Nistal Javier Casqueiro Jose G. Ruiz de Morales
- چاپ و سال / کشور: 2011
Description
Background Intraepithelial lymphocytes (IEL) are a heterogeneous population of lymphocytes raised in celiac disease (CD), whose role in CD pathogenesis remains to be defined. Aims To investigate how the age of diagnosis, diet, and the severity of the histological lesions are related to the changes observed in unconventional IEL populations. Methods Prospective analysis of 101 confirmed celiac patients from a single center, including 66 at diagnosis (45 children, 21 adults) and 112 non-celiac controls (12 children, 100 adults). IEL from duodenal biopsies were studied by six-color flow cytometry. The results were analyzed in relationship with age, diet (gluten intake), and histopathology (Marsh type). Results In comparison with respective age controls, both children and adult patients showed duodenal intraepithelial lymphocytosis with significant differences in every single non-conventional IEL population: CD3? TCR cd, NK (CD3-, CD16?, CD56?), NKT (CD3?, CD161?, CD56?), and iNKT (CD3? Va24) (P\0.001 for all). Gluten intake was not only directly associated with severe atrophy, but also with decreased percentages of NK (P = 0.02), NKT (P = 0.003), and iNKT (P = 0.03). Changes in iNKT and cd IEL were more marked in celiac children compared with celiac adults (P = 0.02 and 0.01, respectively). In contrast, increased CD3? TCR cd were diet- and Marsh grade-independent. Conclusions The typical phenotypical profile of intraepithelial lymphocytosis in untreated pediatric and adult celiacs consists of increased CD3? TCR cd populations with decreased NK, NKT, and iNKT cells. NK, NKT, and iNKT IEL, but not cd IEL, are dynamic populations associated with diet, age, and histopathology.
Dig Dis Sci (2011) 56:2042–2049 DOI 10.1007/s10620-010-1534-5 Received: 11 September 2010 / Accepted: 10 December 2010 / Published online: 8 January 2011
