The Neutrophil Respiratory Burst and Bacterial Digestion in Crohn’s Disease
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Bu’Hussain Hayee Farooq Z. Rahman Jane Tempero Sara McCartney Stuart L. Bloom Anthony W. Segal Andrew M. Smith
- چاپ و سال / کشور: 2010
Description
Background Neutrophils are a key part of the innate immune defence against microbes, using the respiratory burst (RB) to optimise killing and digestion. Previous studies of the neutrophil RB in Crohn’s disease (CD) have yielded conflicting results. Methods Superoxide production in response to phorbolmyristyl acetate (PMA) was measured in neutrophils from 100 patients with CD compared to 50 healthy controls (HCs) and 50 patients with ulcerative colitis (UC). A further 22 CD and 10 HCs were studied using f-Met-Leu-Phe (fMLP), and digestion of E. coli by neutrophils was also evaluated. Results The mean ± SEM PMA-stimulated RB (nmol O2/106 cells/min) was 10.86 ± 0.26 in HCs, 9.76 ± 0.23 in CD (P=0.02) and 10.04 ± 0.28 in UC (P=0.09 vs HC and 0.47 vs CD). No significant effect of age, gender or medication was observed. The RB in three patients with presumed CD was found to be in the range expected in patients with inherited neutrophil disorders. Stimulation with fMLP was calcium dependent and attenuated in patients on 5-ASA. Digestion of E. coli by neutrophils was not different in HC vs CD (21.6 vs 20.53%, P=0.60). Conclusion The significant reduction in neutrophil RB in CD does not appear to result in defective bacterial digestion and is therefore unlikely play a major role in pathogenesis. Three patients in this cohort of patients with presumed idiopathic CD were found to have a profound defect of the neutrophil RB. A high index of suspicion for such patients is prudent, as their prognosis can be improved by altering or augmenting the conventional treatment regimens employed for CD.
Dig Dis Sci (2011) 56:1482–1488 DOI 10.1007/s10620-010-1426-8 Received: 12 March 2010 / Accepted: 9 September 2010 / Published online: 9 October 2010
