Laryngeal transplantation in minipigs: vascular, myologic and functional outcomes
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : M. A. Birchall · P. J. Kingham · P. J. Murison · S. M. Ayling · R. Burt · L. Mitchard · A. Jones · P. Lear · C. R. Stokes · G. Terenghi · M. Bailey ·
- چاپ و سال / کشور: 2011
Description
There is no eVective way of replacing all the functions of the larynx in those requiring laryngectomy. Regenerative medicine oVers promise, but cannot presently deliver implants with functioning neuromuscular units. A single well-documented laryngeal transplant in man was a qualiWed success, but more information is required before clinical trials may be proposed. We studied the early response of the larynx to laryngeal transplantation between 17 pairs of NIH minipigs full matched at the MHC2 locus. Following iterative technical improvements, pigs had good swallowing and a patent airway at 1 week. No signiWcant changes in mucosal blood Xux were observed compared with pre-operative measurements. Changes in muscle morphology and Wbre phenotype were observed in transplant muscles retrieved after 7 days: the levels of fast and slow myosin heavy chain (MyHC) protein were reduced and embryonic MyHC was up regulated consistent with denervation induced atrophy. At 1 week laryngeal transplantation can result in good swallowing, and is not associated with clinical evidence of ischemia-reperfusion injury in MHC-matched pigs.
Eur Arch Otorhinolaryngol (2011) 268:405–414, Received: 9 April 2010 / Accepted: 25 July 2010 / Published online: 15 September 2010 © Springer-Verlag 2010
