Reduction of cisplatin ototoxicity in rats by oral administration of pomegranate extract
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Zahide Mine Yazici · Aysenur Meric · Ahmet Midi · Yasar Volkan Arآnc · Volkan Kahya · Gunter Hafآz
- چاپ و سال / کشور: 2011
Description
The aim of this study was to investigate the eVectiveness of the oral administration of pomegranate extract (PE) as a protective agent against cisplatin-induced ototoxicity. The study included a prospective, controlled animal study Group 1 (n = 6), received no cisplatin or PE, and group 2 (n = 6) received cisplatin at 8 mg/kg/day for 3 consecutive days. Group 3 (n = 6) received not only cisplatin at 8 mg/kg/day for 3 consecutive days, but also received PE (100 L/day) via gavage for 5 days prior to the cisplatin injection and for 3 days concomitantly with the cisplatin injections. To measure cisplatin ototoxic eVects, “distortion product otoacoustic emissions” (DPOAE) were analyzed 3 days before and after the cisplatin injections. Histological changes in the cochleas were observed by light microscopy. Compared with group 3, the DPOAE amplitudes of group 2 decreased signiWcantly. Among the groups, there was a statistically signiWcant diVerence in basal and mid turn external ciliated cells (ECC) number, but there was no statistically signiWcant diVerence in apical turn. DiVerences in stria vascularis (SV) changes were statistically signiWcant between the groups, and the median score for SV injury was signiWcantly greater in group 2 than in group 3. DiVerences in the median scores for SGC changes being signiWcantly greater in group 2 than in group 3. In conclusion, these results indicated that oral administration of PE aVorded statistically signiWcant protection to the cochlea in rats from cisplatin toxicity, and thus, oral experimental dose of PE administration may have a protective eVect against cisplatin ototoxicity in rats.
Eur Arch Otorhinolaryngol DOI 10.1007/s00405-011-1582-2,Received: 4 January 2011 / Accepted: 14 March 2011
