HPV genotypes in the oral cavity/oropharynx of children and adolescents: cross-sectional survey in Poland
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Julia Durzyٌska & Joanna Pacholska-Bogalska & Maria Kaczmarek & Tomasz Hanو & Magdalena Durda & Magdalena Skrzypczak & Anna Goںdzicka-Jَzefiak
- چاپ و سال / کشور: 2011
Description
Human papillomaviruses (HPVs) are a very complex group of pathogenic viruses, with more than 80 types, causing human infection. Given the prevalence of HPV infection and its relationship with the development of cervical and many other cancers, HPV vaccine development has been a major public health initiative worldwide in the last decade. The aim of the presented study was to identify HPV DNA by MY-PCR in 4,150 school children and adolescents, aged 10–18 years in the Wielkopolska region, Poland. All individuals were asked to fill in extensive questionnaires; further normal, oral squamous cells were collected from each pupil. Cellular DNA was isolated and used as a MY-PCR template to estimate the incidence of HPV-active infection. Forty five subjects (1.08% of the sample) were carriers of oropharyngeal HPVs. HPV status and variables of interest, such as age, gender, socioeconomical status, and risk factors (smoking and sexual intercourse history, alcohol consumption) were not correlated. The presence of HPVs in the oral cavity was cumulated in several schools of the region. DNA sequencing of MY-PCR products revealed only four HPV genotypes. The most frequent genotype was HPV11 (38/45 HPV-positive cases), while other more rare genotypes were HPV6 (3/45), HPV12 (3/45), and HPV57 (1/45). Conclusion: Our findings presented herein, reveal a relatively low prevalance of oropharyngeal HPVs in Polish adolescents and fill an important gap in the knowledge of oral HPV infections of children above 10 years and adolescents.
Eur J Pediatr (2011) 170:757–761 DOI 10.1007/s00431-010-1345-x, Received: 27 July 2010 / Accepted: 3 November 2010 / Published online: 24 November 2010
