Muscle regeneration is undisturbed by repeated polytraumatic injury
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : D. B. Horva´thy P. P. Nardai T. Major K. Schandl A. Cselenya´k G. Va´cz L. Kiss M. Szendr}oi Z. Lacza
- چاپ و سال / کشور: 2010
Description
Introduction Clinical observations suggest that repeated injury within a week after a traumatic event impairs the regeneration of tissues. Our aim was to investigate the effect of repeated trauma on the proliferation of satellite cells in skeletal muscle tissue. Materials and methods Cold lesion injury was performed in the soleus muscle and in the motor cortex of anesthetized male Wistar rats 0, 1, or 2 times with 7 day intervals between the interventions. Following the last operation, 5-bromo-20-deoxyuridine was injected i.p. for 6 or 12 days to label dividing cells. Gut epithelium was used as positive control. Immunohistochemistry was performed 1 and 5 weeks after the last injury and the sections were analyzed with confocal microscopy. Results In the case of repeated trauma, the percentage of proliferating cells remained the same compared to single hit animals after 1 week (28.0 ± 2.5% and 29.6 ± 3.0%) as well as after 5 weeks (13.9 ± 1.8% and 14.5 ± 2.2%). Conclusion The second hit phenomenon is probably due to systemic factors rather than to a diminished regenerating potential of injured soft tissues.
Eur J Trauma Emerg Surg (2011) 37:161–167 DOI 10.1007/s00068-010-0034-9 Received: 24 January 2010 / Accepted: 31 May 2010 / Published online: 8 July 2010
