A model system for prediction of the in vivo metabolism of designer drugs using three-dimensional culture of rat and human hepatocytes
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Tatsuyuki Kanamori Kenji Kuwayama Kenji Tsujikawa Hajime Miyaguchi Yuko Togawa-Iwata Hiroyuki Inoue
- چاپ و سال / کشور: 2011
Description
The in vitro metabolisms of 4-bromo-2,5- dimethoxyphenethylamine (2C-B), 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), and 2,5-dimethoxy-4- propylthiophenethylamine (2C-T-7) were studied using TESTLIVERTM-rat and TESTLIVERTM-human, which are new three-dimensional rat and human hepatocyte culture systems, respectively. The metabolites produced in the incubation media were measured by liquid chromatography– tandem mass spectrometry or gas chromatography–mass spectrometry. The data obtained with the in vitro system of rat origin were carefully compared with those obtained by rat in vivo experiments; most of themetabolites found in the in vivo experiments could be reproduced in the present threedimensional in vitro experiments, although quantitative metabolite distribution patterns obtained with the threedimensional system was somewhat different from those of in vivo experiments. Because human in vivo experiments are not possible, especially for dubious designer drugs, the in vitro experiments using the three-dimensional human hepatocyte culture system seem very useful for studying human metabolism of new designer drugs as an alternative to human experiments.
Forensic Toxicol (2011) 29:142–151 DOI 10.1007/s11419-011-0116-3 Received: 25 April 2011 / Accepted: 18 May 2011 / Published online: 4 June 2011
