Functional restoration of endothelial cells of the cryopreserved heart valve
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Eiki Fujimoto, MD · Masanori Yoshizumi, MD, PhD Tamotsu Kanbara, MD · Hirotsugu Kurobe, MD, PhD Tatsuo Motoki, MD · Mikio Sugano, MD Taisuke Nakaya
- چاپ و سال / کشور: 2010
Description
Purpose. Although several approaches have been tried to improve the durability of cryopreserved valves, cellular restoration after thawing remains to be investigated. The aim of our study was to assess the functional restoration of endothelial cells of cryopreserved heart valves by in vitro culture for an alternative step to improving longevity. Methods. Cryopreserved human umbilical vein endothelial cells (HUVECs) and porcine aortic cusps were cultivated for 14 days after thawing. Then the cellular activity of the enzymes cytosolic esterase and mitochondrial dehydrogenase was measured. The cellular viability of cryopreserved cusps was also assessed using confocal laser scanning microscopy. Results. The number of viable HUVECs decreased markedly after cryopreservation and thawing but recovered to pre-cryopreservation level after 14 days of culture. In contrast, the enzyme activity of the cryopreserved porcine aortic cusps showed recovery at 7 days of in vitro tissue culture after thawing. Confocal laser scanning microscopy fi ndings showed that the cellular cytosolic esterase activity of cryopreserved cusps deterioratedafter thawing but displayed considerable recovery by day 14 of culture. Conclusion. The functional recovery of endothelial cells in cryopreserved heart valves seems to require tissue culture of at least 14 days. Ex vivo endothelial restoration of cryopreserved heart valves may add to heart valve durability.
Gen Thorac Cardiovasc Surg (2011) 59:169–174 DOI 10.1007/s11748-010-0711-y Received: 22 June 2010 / Accepted: 7 September 2010
