Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : B. N. Rooban & V. Sasikala & V. Sahasranamam & Annie Abraham
- چاپ و سال / کشور: 2011
Description
Purpose Recent evidence suggests that oxidative stress plays a significant role in the development of cataract. The present study sought to evaluate the efficacy of flavonoid fraction of Vitex negundo (FVN) in preventing the toxicity induced by sodium selenite in vitro culture condition. Methods Enucleated rat lenses were maintained in organ culture containingDMEMmediumalone (G I), supplemented with 0.1mM sodium selenite (G II), sodium selenite + 20 ìg/ml quercetin (G III) and sodium selenite + 50 ìg/ml FVN (G IV). Treatment was from the second to fifth day, while selenite administration was done on the third day. After the experimental period, lenses were taken out and the activities of superoxide dismutase (SOD), catalase, Ca2+ ATPase, levels of reduced glutathione (GSH), calcium, reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and sulfhydryl content were studied. Results Morphological examination revealed dense vacuolization and loss of cortical transparency in G II compared to control and treated group. The mean activities of the enzymes SOD, catalase and Ca2+ ATPase, levels of GSH and sulfhydryl content were significantly reduced in lenses of G II compared to control. In addition, the mean levels of ROS, calcium and TBARS were elevated in G II compared to control. However, these changes were modulated by FVN treatment to further strengthen its protective role over selenite cataract. Conclusion These results suggest that FVN treatment prevented selenite toxicity and cataractogenesis by maintaining antioxidant status, calcium homeostasis, protecting sulfhydryl group, and decreasing oxidative stress in lens, which may be due to its protective effects.
Graefes Arch Clin Exp Ophthalmol (2011) 249:685–692 DOI 10.1007/s00417-010-1598-0 Received: 20 July 2010 / Revised: 1 November 2010 / Accepted: 2 December 2010 / Published online: 15 January 2011
