B and T lymphocyte attenuator regulates the development of antigen-induced experimental conjunctivitis
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Waka Ishida & Ken Fukuda & Mina Kajisako & Tamaki Sumi & Hironori Matsuda & Hideo Yagita & Atsuki Fukushima
- چاپ و سال / کشور: 2011
Description
Purpose To investigate the roles that B and T lymphocyte attenuator (BTLA) and herpesvirus entry mediator (HVEM) play in the development of antigen-induced experimental conjunctivitis (EC). Methods BALB/c mice were immunized with ragweed (RW) in alum. Ten days later, the mice were challenged with RW in eye drops. After 24 hours, the conjunctivas, blood and spleens were collected for histological analysis, measurement of serum immunoglobulin (Ig) levels, and both flow cytometric analysis and cytokine assays, respectively. The mice were injected intraperitoneally with anti- BTLA antibody, anti-HVEM antibody or control antibody during either induction phase or effector phase. Results Induction-phase treatment with anti-BTLA antibody but not anti-HVEM antibody significantly increased conjunctival eosinophil infiltration. Treatment with either antibody during the effector phase did not affect conjunctival eosinophil infiltration. Anti-BTLA antibody treatment during the induction phase reduced the B cell compartment and increased the CD11b-positive cell compartment in splenocytes. Additionally, anti-BTLA treatment upregulated IL-4 and IL-10 production of splenocytes stimulated by RW. Conclusions BTLA regulated the development of EC possibly by downregulating Th2 cytokine production and adjusting the compartments of immunocompetent cells. The regulation of EC by BTLA may be mediated by BTLA ligands other than HVEM
Graefes Arch Clin Exp Ophthalmol DOI 10.1007/s00417-011-1695-8 Received: 19 February 2011 / Revised: 6 April 2011 / Accepted: 10 April 2011
