Differentiation profile of peripheral blood-derived vascular progenitor cell predicts intimal hyperplasia after coronary stenting
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Chao-Hung Wang I-Chang Hsieh Wen-Jin Cherng Chun-Chi Chen Tao-Hsin Tung Ju-Fang Lee Shing-Jong Lin Po-Nan Wang
- چاپ و سال / کشور: 2011
Description
In-stent restenosis is largely due to intimal hyperplasia (IH). The number of vascular progenitor cells (VPCs) mobilized at the acute phase after stenting is associated with IH. This study sought to determine whether the differentiation profile of VPC predicts the development of IH. Peripheral blood was collected in 58 patients after bare-metal stenting to culture VPCs. Intravascular ultrasound was performed to estimate the area of IH 6 months after stenting. VPC differentiation was determined using flow cytometry. VE-cadherin (VE-Cad) and a-smooth muscle actin (a-SMA) were used to identify endothelial and smooth muscle cell lineages, respectively. After culturing, VPCs differentiated into four different phenotypes (a-SMA-VE-Cad?, a-SMA?VE-cadhigh, a-SMA?VE-cadlow, and a-SMA?VE-Cad-). IH was correlated with gender (P = 0.04), smoking status (P = 0.04), reference diameter (P = 0.03), minimal lumen diameter (P = 0.03), stent area (P\0.0001), and parameters in the VPC differentiation profile (P\0.05). Multivariate analysis controlling for stent area, smoking status, and gender revealed that IH was positively and independently associated with the number of differentiated a-SMA?VE-Cad low/- VPCs (P\0.0001), and the ratio of a-SMA?VE-Cad low/- VPCs to a-SMA-VE-Cad? VPCs (P = 0.001). These parameters in the VPC differentiation profile independently predicted the IH and provided additive information to traditional risk factors. In conclusion, the profile of VPC differentiation predicts the severity of post-stent IH and may be a potential tool in the future for clinicians to identify patients at risk of post-stent restenosis.
Heart Vessels DOI 10.1007/s00380-011-0118-4 Received: 1 September 2010 / Accepted: 14 January 2011
