Agonist-like autoantibodies against calcium channel in patients with dilated cardiomyopathy
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Hua Xiao Min Wang Yimei Du Jing Yuan Guanghong Zhao Danna Tu Yu-Hua Liao
- چاپ و سال / کشور: 2011
Description
The calcium channel may be an important target in the autoimmune pathogenesis of dilated cardiomyopathy (DCM). The presence and function of serum autoantibodies against calcium channels (CC-AAbs) in patients with DCM were studied. Calcium-channel AAbs were detected in 80 patients with DCM and 80 controls by enzyme-linked immunosorbent assay (ELISA). Calciumchannel AAbs were further purified by affinity chromatography for characterization by immunoblotting and immunofluorescence. Voltage-clamp experiments were performed to identify the function of CC-AAbs. The presence of CC-AAbs was shown effectively by ELISA, and CC-AAbs were able specifically to bind to the calcium channel on the myocyte, confirmed by immunoblotting and immunofluorescence. Calcium currents were enhanced by CC-AAbs on Xenopus oocytes expressing human CaV1.2 channels, which suggested CC-AAbs in patients with DCM were agonist-like. Our results suggest there are novel agonist-like CC-AAbs in patients with DCM. Calciumchannel AAbs might play an important role in the pathogenesis of DCM.
Heart Vessels DOI 10.1007/s00380-011-0176-7 Received: 29 September 2010 / Accepted: 8 July 2011
