The effect of a single episode of antimicrobial photodynamic therapy in the treatment of experimental periodontitis. Microbiological profile and cytokine pattern in the dog mandible
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Rafael Ramos de Oliveira & Arthur Belém Novaes Jr. & Gustavo P. Garlet & Raphael F. de Souza & Mلrio Taba Jr. & Sandra Sato & Sérgio L. S. de Souza &
- چاپ و سال / کشور: 2011
Description
The purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (aPDT) on microbiological profile and cytokine pattern in dogs. Periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. The dogs were randomly treated with aPDT using a dye/laser system, scaling and root planning (SRP), or with the association of treatments (SRP+aPDT). Plaque samples were collected at baseline, 1, 3, and 4 weeks, and the mean counts of 40 species were determined using DNA-DNA hybridization. Gingival biopsies were removed and the expression of tumor necrosis factor alpha (TNF-ل), receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase (MMP-1), interleukin (IL) 6, IL-10 and total bacterial load by analysis of 16 S rRNA gene were evaluated through real-time PCR. The results shows that the levels of the majority of the species were reduced 1 week post-therapy for all treatments, however, an increase in counts of Prevotella intermedia (p=0.00), Prevotella. nigrescens (p=0.00) and Tannerella forsythia (p=0.00) was observed for aPDT and SRP+aPDT. After 4 weeks, a regrowth of Porphyromonas gingivalis (p=0.00) and Treponema denticola (p=0.00), was observed for all treatments. Also, a strikingly reduction of counts on counts of Aggregatibacter actinomycetemcomitans was observed for the aPDT (p=0.00). For the cytokine pattern, the results were similar for all treatments, and a reduction in the expression of cytokines and bacterial load was observed throughout the study. Our results suggest that SRP, aPDT in a single application, and SRP+aPDT affects different bacterial species and have similar effects on the expression of cytokines evaluated during the treatment of ligature-induced periodontitis.
Lasers Med Sci (2011) 26:359–367 DOI 10.1007/s10103-010-0864-z Received: 1 February 2010 / Accepted: 1 November 2010 / Published online: 18 November 2010
