Influence of creatine supplementation on bone quality in the ovariectomized rat model: an FT-Raman spectroscopy study
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Renato Aparecido de Souza & Murilo Xavier & Fabiano Fernandes da Silva & Marco Tْlio de Souza & Maira Gaspar Tosato & Airton Abrahمo Martin & Julio
- چاپ و سال / کشور: 2011
Description
The influence of creatine (Cr) supplementation on cortical and trabecular bone from ovariectomized rats was studied using FT-Raman spectroscopy. The intensity of organic-phase Raman bands was compared to mineral phase ones. Twenty-one female Wistar rats aged 3 months were divided into three groups (n=7 per group): ovariectomized (OVX), ovariectomized treated with creatine (CRE) and sham-operated (SHAM) groups. Creatine supplementation (300 mg kg-1 day-1) was provided for 8 weeks, starting 12 weeks after ovariectomy. FT-Raman spectroscopy was performed on the right medial femoral mid-shaft (cortical bone) and third lumbar vertebral body (trabecular bone). The integrated intensities of mineral phase (phosphate and carbonate bands at 959 and 1,071 cm-1, respectively) and organic phase (amide I band at 1,665 cm-1) Raman bands were analyzed. The mineralto- matrix (phosphate/amide I), carbonate-to-phosphate, and carbonate-to-amide I ratios were analyzed to assess bone quality. The phosphate content on trabecular bone was higher in the CRE group than the OVX group (p<0.05). No significant changes in mineral or organic phases on cortical bone were observed. A radiographic assessment of bone density was encouraging as the same findings were showed by Raman intensity of phosphate from cortical (r2=0.8037) and trabecular bones (r2=0.915). Severe ovariectomyinduced bone loss was confirmed by FT-Raman spectroscopy. The results suggest that the chemical composition of trabecular bone tissue may be positively influenced by Cr supplementation after ovariectomy.
Lasers Med Sci DOI 10.1007/s10103-011-0976-0 Received: 28 April 2011 / Accepted: 15 July 2011
