Inflammatory cytokines are suppressed by light-emitting diode irradiation of P. gingivalis LPS-treated human gingival fibroblasts Inflammatory cytokine changes by LED irradiation
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : HongRan Choi &WonBong Lim & InAe Kim & JiSun Kim & YoungJong Ko & HyukIl Kwon & SangWoo Kim & K. M. Ahsan Kabir & Xiaojie Li & Oksu Kim & YoungJoon
- چاپ و سال / کشور: 2011
Description
Human gingival fibroblasts (hGFs) play an important role in the inflammatory reaction to lipopolysaccharide (LPS) from P. gingivalis, which infects periodontal connective tissue. In addition, although light-emitting diode (LED) irradiation has been reported to have biostimulatory effects, including anti-inflammatory activity, the pathological mechanisms of these effects are unclear. This study examined the effects of 635-nm irradiation of P. gingivalis LPS-treated human gingival fibroblasts on inflammatory cytokine profiles and the mitogen-activated protein kinase (MAPK) pathway, which is involved in cytokine production. Gingival fibroblasts treated or not treated with P. gingivalis LPS were irradiated with 635-nm LED light, and cytokine profiles in the supernatant were assessed using a human inflammation antibody array. Expression of cyclooxyginase-2 (COX-2) protein and phosphorylation of extracellular signal-regulated kinase (ERK 1/2), p38, and c-Jun-N-terminal kinase (JNK) were assessed by Western-blot analysis to determine the effects on the MAPK pathway, and prostaglandin E2 (PGE2) in the supernatant was measured using an enzyme-linked immunoassay. COX-2 protein expression and PGE2 production were significantly increased in the LPS-treated group and decreased by LED irradiation. LPS treatment of gingival fibroblasts led to the increased release of the proinflammatory- related cytokines interleukin-6 (IL-6) and IL- 8, whereas LED irradiation inhibited their release. Analysis of MAPK signal transduction revealed a considerable decrease in p38 phosphorylation in response to 635-nm radiation either in the presence or absence of LPS. In addition, 635-nm LED irradiation significantly promoted JNK phosphorylation in the presence of LPS. LED irradiation can inhibit activation of pro-inflammatory cytokines, mediate the MAPK signaling pathway, and may be clinically useful as an anti-inflammatory tool.
Lasers Med Sci DOI 10.1007/s10103-011-0971-5 Received: 16 July 2010 / Accepted: 12 July 2011